{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T20:09:56Z","timestamp":1787774996101,"version":"build-2784847793"},"reference-count":44,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2020,3,29]],"date-time":"2020-03-29T00:00:00Z","timestamp":1585440000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004763","name":"Natural Science Foundation of Inner Mongolia","doi-asserted-by":"publisher","award":["2019MS01013"],"award-info":[{"award-number":["2019MS01013"]}],"id":[{"id":"10.13039\/501100004763","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>During an outbreak of respiratory diseases including atypical pneumonia in Wuhan, a previously unknown \u03b2\u2010coronavirus was detected in patients. The newly discovered coronavirus is similar to some \u03b2\u2010coronaviruses found in bats but different from previously known SARS\u2010CoV and MERS\u2010CoV. High sequence identities and similarities between 2019\u2010nCoV and SARS\u2010CoV were found. In this study, we searched the homologous templates of all nonstructural and structural proteins of 2019\u2010nCoV. Among the nonstructural proteins, the leader protein (nsp1), the papain\u2010like protease (nsp3), the nsp4, the 3C\u2010like protease (nsp5), the nsp7, the nsp8, the nsp9, the nsp10, the RNA\u2010directed RNA polymerase (nsp12), the helicase (nsp13), the guanine\u2010N7 methyltransferase (nsp14), the uridylate\u2010specific endoribonuclease (nsp15), the 2'\u2010O\u2010methyltransferase (nsp16), and the ORF7a protein could be built on the basis of homology templates. Among the structural proteins, the spike protein (S\u2010protein), the envelope protein (E\u2010protein), and the nucleocapsid protein (N\u2010protein) can be constructed based on the crystal structures of the proteins from SARS\u2010CoV. It is known that PL\u2010Pro, 3CL\u2010Pro, and RdRp are important targets for design antiviral drugs against 2019\u2010nCoV. And S protein is a critical target candidate for inhibitor screening or vaccine design against 2019\u2010nCoV because coronavirus replication is initiated by the binding of S protein to cell surface receptors. It is believed that these proteins should be useful for further structure\u2010based virtual screening and related computer\u2010aided drug development and vaccine design.<\/jats:p>","DOI":"10.1002\/jmv.25768","type":"journal-article","created":{"date-parts":[[2020,3,17]],"date-time":"2020-03-17T13:57:13Z","timestamp":1584453433000},"page":"1542-1548","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":91,"title":["A guideline for homology modeling of the proteins from newly discovered betacoronavirus, 2019 novel coronavirus (2019\u2010nCoV)"],"prefix":"10.1002","volume":"92","author":[{"given":"Shengjie","family":"Dong","sequence":"first","affiliation":[{"name":"Faculty of Education and Sports Guangdong Baiyun University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiachen","family":"Sun","sequence":"additional","affiliation":[{"name":"School of Biotechnology and Food Science Tianjin University of Commerce Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuo","family":"Mao","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Low Dimensional Materials Physics and Preparing Technology, Department of Applied Physics Institute of Advanced Materials Physics, Faculty of Science, Tianjin University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Science Inner Mongolia University of Science &amp; Technology Baotou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Lin","family":"Lu","sequence":"additional","affiliation":[{"name":"College of New Energy Bohai University Jinzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5230-2874","authenticated-orcid":false,"given":"Jiesen","family":"Li","sequence":"additional","affiliation":[{"name":"School of Environment and Chemical Engineering Foshan University Foshan China"},{"name":"Department of Research and Development Guangzhou Ginpie Technology Co., Ltd. Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,3,29]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa030747"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1211721"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30183-5"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30154-9"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30185-9"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30251-8"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30211-7"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2001017"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMe2001329"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMp2000929"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMe2001126"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2001316"},{"key":"e_1_2_8_14_1","unstructured":"https:\/\/www.ncbi.nlm.nih.gov\/nuccore\/MN908947"},{"key":"e_1_2_8_15_1","unstructured":"https:\/\/www.ncbi.nlm.nih.gov\/nuccore\/MN988713"},{"key":"e_1_2_8_16_1","unstructured":"https:\/\/www.ncbi.nlm.nih.gov\/nuccore\/MN994468"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11427-020-1637-5"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abb0659"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2001191"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature17180"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aal3653"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aau9242"},{"key":"e_1_2_8_23_1","first-page":"1","article-title":"COVID\u20102019: the role of the nsp2 and nsp3 in its pathogenesis","author":"Angeletti S","year":"2020","journal-title":"J Med Virol"},{"key":"e_1_2_8_24_1","first-page":"1","article-title":"Composition and divergence of coronavirus spike proteins and host ACE2 receptors predict potential intermediate hosts of SARS\u2010CoV\u20102","author":"Liu Z","year":"2020","journal-title":"J Med Virol"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/1745-6150-7-12"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh435"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl164"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn201"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200900140"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btq662"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw1132"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky427"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/msb.2011.75"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.3290"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abb2507"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abb2762"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aba9757"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2020.0757"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2020.3972"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2020.3882"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2020.4031"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2008-3"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2012-7"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.25768","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.25768","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jmv.25768","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.25768","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T03:34:02Z","timestamp":1693884842000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.25768"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3,29]]},"references-count":44,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2020,9]]}},"alternative-id":["10.1002\/jmv.25768"],"URL":"https:\/\/doi.org\/10.1002\/jmv.25768","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,3,29]]},"assertion":[{"value":"2020-02-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-03-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-03-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}