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To identify the biological characteristics of SARS\u2010CoV\u20102 in a normal laboratory environment (biosafety level 2 [BSL\u20102]), a lentiviral\u2010based nucleocapsid was used to carry the spike protein of SARS\u2010CoV\u20102 onto the surface of pseudoviral particles as a surrogate model to evaluate the infective characterization of SARS\u2010CoV\u20102. This study indicated that SARS\u2010CoV\u20102 has extensive tissue tropism for humans and may infect monkeys and tree shrews but not rodents. More importantly, the use of pseudoviral particles in this study allows rapid assessment of neutralizing antibodies in serum in a BSL\u20102 laboratory. This study will provide a quick and easy tool for evaluating neutralizing antibodies in the serum of recovering patients and assessing the potency of candidate vaccines.<\/jats:p>","DOI":"10.1002\/jmv.25865","type":"journal-article","created":{"date-parts":[[2020,4,10]],"date-time":"2020-04-10T10:50:25Z","timestamp":1586515825000},"page":"1609-1614","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Evaluate severe acute respiratory syndrome coronavirus 2 infectivity by pseudoviral particles"],"prefix":"10.1002","volume":"92","author":[{"given":"Tao","family":"Pu","sequence":"first","affiliation":[{"name":"Institute of Medical Biology Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing China"},{"name":"Yunnan Key Laboratory of Vaccine Research and Development of Severe Infectious Disease  Kunming China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen","family":"Ding","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing China"},{"name":"Yunnan Key Laboratory of Vaccine Research and Development of Severe Infectious Disease  Kunming China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yadong","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing China"},{"name":"Yunnan Key Laboratory of Vaccine Research and Development of Severe Infectious Disease  Kunming China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaojuan","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing China"},{"name":"Yunnan Key Laboratory of Vaccine Research and Development of Severe Infectious Disease  Kunming China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haiwei","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing China"},{"name":"Yunnan Key Laboratory of Vaccine Research and Development of Severe Infectious Disease  Kunming China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinmei","family":"Duan","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing China"},{"name":"Yunnan Key Laboratory of Vaccine Research and Development of Severe Infectious Disease  Kunming China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing China"},{"name":"Yunnan Key Laboratory of Vaccine Research and Development of Severe Infectious Disease  Kunming China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanwei","family":"Bi","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing China"},{"name":"Yunnan Key Laboratory of Vaccine Research and Development of Severe Infectious Disease  Kunming China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Cun","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing China"},{"name":"Yunnan Key Laboratory of Vaccine Research and Development of Severe Infectious Disease  Kunming China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,4,25]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"WHO. 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