{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T10:54:54Z","timestamp":1788864894761,"version":"build-2803163510"},"reference-count":26,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,5,17]],"date-time":"2020-05-17T00:00:00Z","timestamp":1589673600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The micro\u2010neutralization assay is a fundamental test in virology, immunology, vaccine assessment, and epidemiology studies. Since the SARS\u2010CoV\u20102 outbreak at the end of December 2019 in China, it has become extremely important to have well\u2010established and validated diagnostic and serological assays for this new emerging virus. Here, we present a micro\u2010neutralization assay with the use of SARS\u2010CoV\u20102 wild type virus with two different methods of read\u2010out. We evaluated the performance of this assay using human serum samples taken from an Italian seroepidemiological study being performed at the University of Siena, along with the human monoclonal antibody CR3022 and some iper\u2010immune animal serum samples against Influenza and Adenovirus strains. The same panel of human samples have been previously tested in enzyme\u2010linked immunosorbent assay (ELISA) as a pre\u2010screening. Positive, borderline, and negative ELISA samples were evaluated in neutralization assay using two different methods of read\u2010out: subjective (by means of an inverted optical microscope) and objective (by means of a spectrophotometer). Our findings suggest that at least 50% of positive ELISA samples are positive in neutralization as well, and that method is able to quantify different antibody concentrations in a specific manner. Taken together, our results confirm that the colorimetric cytopathic effect\u2010based microneutralization assay could be used as a valid clinical test method for epidemiological and vaccine studies.<\/jats:p>","DOI":"10.1002\/jmv.25986","type":"journal-article","created":{"date-parts":[[2020,5,8]],"date-time":"2020-05-08T07:58:46Z","timestamp":1588924726000},"page":"2096-2104","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":175,"title":["Evaluation of SARS\u2010CoV\u20102 neutralizing antibodies using a CPE\u2010based colorimetric live virus micro\u2010neutralization assay in human serum samples"],"prefix":"10.1002","volume":"92","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4027-7296","authenticated-orcid":false,"given":"Alessandro","family":"Manenti","sequence":"first","affiliation":[{"name":"VisMederi Research s.r.l.  Siena Italy"},{"name":"VisMederi s.r.l.  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Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia M.","family":"Trombetta","sequence":"additional","affiliation":[{"name":"Department of Molecular and Developmental Medicine University of Siena  Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serena","family":"Marchi","sequence":"additional","affiliation":[{"name":"Department of Molecular and Developmental Medicine University of Siena  Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuele","family":"Montomoli","sequence":"additional","affiliation":[{"name":"VisMederi Research s.r.l.  Siena Italy"},{"name":"VisMederi s.r.l.  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