{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T13:28:48Z","timestamp":1791379728892,"version":"4.3.3"},"reference-count":20,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2020,6,2]],"date-time":"2020-06-02T00:00:00Z","timestamp":1591056000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","award":["5\/8\/7\/2020\/ECD\u20101"],"award-info":[{"award-number":["5\/8\/7\/2020\/ECD\u20101"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>One major advantage of molecular assays for human papillomavirus (HPV) DNA detection is that these assays can be performed on self\u2010collected samples unlike cytology or visual inspection with acetic acid (VIA). This cross\u2010sectional study was carried out between March 2017 and April 2019 to compare the diagnostic performance in self\u2010collected urine and vaginal samples for HPV DNA detection. Viral DNA was extracted from processed samples using a\u00a0Qiagen viral DNA extraction Kit (QIAamp DNA Mini Kit). To detect four common high\u2010risk HPV types (16, 18, 31, 45), multiplex real\u2010time polymerase chain reaction (PCR) targeting the LCR\/E6\/E7 region of the HPV genome was performed in ABI 7500 cycler (Applied Biosystems). The negative samples were screened by conventional PCR targeting the L1 capsid region to exclude other HPV types. The overall agreement between the two self\u2010collecting sampling methods was 64.04% with a <jats:italic>\u03ba<\/jats:italic> value of 0.29 pointing towards a fair agreement (<jats:italic>P<\/jats:italic>\u2009&lt;\u2009.01). The sensitivity of HPV DNA detection in urine samples was 57.95% (47.52%, 67.72), and specificity was 84.6% (66.47%, 93.85%) when compared with vaginal samples. The study concludes that self\u2010collected vaginal HPV DNA testing is more sensitive than unpreserved\u2010urine samples for HPV DNA detection in a hospital\u2010based setting.<\/jats:p>","DOI":"10.1002\/jmv.26043","type":"journal-article","created":{"date-parts":[[2020,5,21]],"date-time":"2020-05-21T12:57:44Z","timestamp":1590065864000},"page":"3884-3888","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Comparison of two self\u2010sampling methods for human papillomavirus (HPV) DNA testing among women with high prevalence rates"],"prefix":"10.1002","volume":"92","author":[{"given":"Santhosh","family":"Kuriakose","sequence":"first","affiliation":[{"name":"Gynecologic Oncology Division, Department of Obstetrics and Gynecology Government Medical College Kozhikode Kerala India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2099-9312","authenticated-orcid":false,"given":"Sasidharanpillai","family":"Sabeena","sequence":"additional","affiliation":[{"name":"Manipal Institute of Virology, Manipal Academy of Higher Education Manipal Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Binesh","family":"Damodaran","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology Government Medical College Kozhikode Kerala India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Ravishankar","sequence":"additional","affiliation":[{"name":"Department of Data Science Manipal Academy of Higher Education (MAHE) Manipal Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amrutha","family":"Ramachandran","sequence":"additional","affiliation":[{"name":"Gynecologic Oncology Division, Department of Obstetrics and Gynecology Government Medical College Kozhikode Kerala India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nurul","family":"Ameen","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Government Medical College Kozhikode Kerala India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,6,2]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.k4823"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.4103\/ijmr.IJMR_5_17"},{"key":"e_1_2_10_4_1","unstructured":"ICO\/IARC Information Centre on HPV and Cancer (HPV Information Centre). 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