{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T04:39:57Z","timestamp":1793248797852,"version":"4.5.0"},"reference-count":16,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,6,19]],"date-time":"2020-06-19T00:00:00Z","timestamp":1592524800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    In this study, we designed a set of SARS\u2010CoV\u20102 enrichment probes to increase the capacity for sequence\u2010based virus detection and obtain the comprehensive genome sequence at the same time. This universal SARS\u2010CoV\u20102 enrichment probe set contains 502 120\u2009nt single\u2010stranded DNA biotin\u2010labeled probes designed based on all available SARS\u2010CoV\u20102 viral sequences and it can be used to enrich for SARS\u2010CoV\u20102 sequences without prior knowledge of type or subtype. Following the CDC health and safety guidelines, marked enrichment was demonstrated in a virus strain sample from cell culture, three nasopharyngeal swab samples (cycle threshold [\n                    <jats:italic>C<\/jats:italic>\n                    <jats:sub>t<\/jats:sub>\n                    ] values: 32.36, 36.72, and 38.44) from patients diagnosed with COVID\u201019 (positive control) and four throat swab samples from patients without COVID\u201019 (negative controls), respectively. Moreover, based on these high\u2010quality sequences, we discuss the heterozygosity and viral expression during coronavirus replication and its phylogenetic relationship with other selected high\u2010quality samples from the Genome Variation Map. Therefore, this universal SARS\u2010CoV\u20102 enrichment probe system can capture and enrich SARS\u2010CoV\u20102 viral sequences selectively and effectively in different samples, especially clinical swab samples with a relatively low concentration of viral particles.\n                  <\/jats:p>","DOI":"10.1002\/jmv.26116","type":"journal-article","created":{"date-parts":[[2020,6,3]],"date-time":"2020-06-03T13:10:30Z","timestamp":1591189830000},"page":"2221-2226","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["High\u2010coverage SARS\u2010CoV\u20102 genome sequences acquired by target capture sequencing"],"prefix":"10.1002","volume":"92","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1223-4720","authenticated-orcid":false,"given":"Shaoqing","family":"Wen","sequence":"first","affiliation":[{"name":"Guangzhou Regenerative Medicine and Health Guangdong Laboratory Guangzhou China"},{"name":"Institute of Archaeological Science, School of Life Sciences Fudan University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang","family":"Sun","sequence":"additional","affiliation":[{"name":"Institute of Archaeological Science, School of Life Sciences Fudan University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huanying","family":"Zheng","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Center for Disease Control and Prevention Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingxiang","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Archaeological Science, School of Life Sciences Fudan University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Center for Disease Control and Prevention Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lirong","family":"Zou","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Center for Disease Control and Prevention Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhe","family":"Liu","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Center for Disease Control and Prevention Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Panxin","family":"Du","sequence":"additional","affiliation":[{"name":"Institute of Archaeological Science, School of Life Sciences Fudan University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuding","family":"Xu","sequence":"additional","affiliation":[{"name":"Institute of Archaeological Science, School of Life Sciences Fudan University Shanghai 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Shenzhen BGI Shenzhen Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Guangzhou Regenerative Medicine and Health Guangdong Laboratory Guangzhou China"},{"name":"CAS Key Laboratory of Regenerative Biology, Joint School of Life Sciences, Guangzhou Institutes of Biomedicine and Health Chinese Academy of Sciences Guangzhou 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