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Investigation of this viral infection in the human bronchial epithelial cell line (16HBE) suggests that SARS\u2010CoV\u20102 can enter these cells through interaction between its membrane\u2010localized S protein with the angiotensin\u2010converting enzyme 2 molecule on the host cell membrane. Further observation indicates distinct viral replication with a dynamic and moderate increase, whereby viral replication does not lead to a specific cytopathic effect but maintains a continuous release of progeny virions from infected cells. Although messenger RNA expression of various innate immune signaling molecules is altered in the cells, transcription of interferons\u2010\u03b1 (IFN\u2010\u03b1), IFN\u2010\u03b2, and IFN\u2010\u03b3 is unchanged. Furthermore, expression of some interleukins (IL) related to inflammatory reactions, such as IL\u20106, IL\u20102, and IL\u20108, is maintained at low levels, whereas that of ILs involved in immune regulation is upregulated. Interestingly, IL\u201022, an IL that functions mainly in tissue repair, shows very high expression. Collectively, these data suggest a distinct infection process for this virus in respiratory epithelial cells, which may be linked to its clinicopathological mechanism.<\/jats:p>","DOI":"10.1002\/jmv.26200","type":"journal-article","created":{"date-parts":[[2020,6,19]],"date-time":"2020-06-19T14:17:55Z","timestamp":1592576275000},"page":"2830-2838","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Distinct infection process of SARS\u2010CoV\u20102 in human bronchial epithelial cell lines"],"prefix":"10.1002","volume":"92","author":[{"given":"Yun","family":"Liao","sequence":"first","affiliation":[{"name":"Institute of Medical Biology, Chinese Academy of Medicine Science &amp; Peking Union Medical College Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases  Kunming Yunnan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueqi","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Medical Biology, Chinese Academy of Medicine Science &amp; 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