{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T07:55:17Z","timestamp":1761897317587,"version":"3.37.3"},"reference-count":15,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,7,6]],"date-time":"2020-07-06T00:00:00Z","timestamp":1593993600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The main purpose of this study is to evaluate the presence of viral RNA of severe acute respiratory syndrome coronavirus\u20102 (SARS\u2010CoV\u20102) in conjunctival swab specimen of coronavirus disease 2019 (COVID\u201019) patients with and without conjunctivitis to establish the diagnostic value of reverse transcription\u2010polymerase chain reaction (RT\u2010PCR) in each case and to describe its clinical characteristics. A cross\u2010sectional study was conducted at the Hospital Clinico San Carlos of Madrid, Spain. Thirty\u2010six subjects from the COVID admission unit with laboratory\u2010confirmed SARS\u2010CoV\u20102 infection were included. Conjunctival swabs were collected from 18 patients with conjunctivitis and 18 patients without conjunctivitis and RT\u2010PCR was performed. Conjunctival swab was collected from both eyes of 36 patients (72 eyes), detecting SARS\u2010CoV\u20102 RNA in conjunctival swab of two patients (5.5%). Among the 18 patients with conjunctivitis, only one of them (5.5%) showed positive results. Likewise, SARS\u2010CoV\u20102 RNA was detected in one patient without conjunctivitis (5.5%). The mean age of the 36 patients was 67.9 years (range, 28\u201092 years) and the male\u2010to\u2010female ratio was 0.44 (16:20). The mean days since the onset of COVID\u201019 symptoms until conjunctivitis manifestation was 8 (range, 1\u201024 days). The mean duration of the conjunctivitis was 3 days (range, 1\u20107 days). SARS\u2010CoV\u20102 RNA may be detected in conjunctival swabs of both patients with and without conjunctivitis. This study revealed the same rate of positive results amongst the group with and without conjunctivitis, suggesting that detecting SARS\u2010CoV\u20102 in ocular fluids is not conditioned on the presence of conjunctivitis. The presence of SARS\u2010CoV\u20102 RNA in ocular samples highlights the role of the eye as a possible route of transmission of the disease.<\/jats:p>","DOI":"10.1002\/jmv.26219","type":"journal-article","created":{"date-parts":[[2020,6,24]],"date-time":"2020-06-24T16:41:27Z","timestamp":1593016887000},"page":"383-388","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["Detecting SARS\u2010CoV\u20102 RNA in conjunctival secretions: Is it a valuable diagnostic method of COVID\u201019?"],"prefix":"10.1002","volume":"93","author":[{"given":"Noemi","family":"G\u00fcemes\u2010Villahoz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2178-6164","authenticated-orcid":false,"given":"Barbara","family":"Burgos\u2010Blasco","sequence":"additional","affiliation":[{"name":"Servicio de Oftalmolog\u00eda, Hospital Cl\u00ednico San Carlos Instituto de Investigaci\u00f3n Sanitaria del Hospital Cl\u00ednico San Carlos (IdISSC)  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Arribi\u2010Vilela","sequence":"additional","affiliation":[{"name":"Department of Microbiology Instituto de Investigaci\u00f3n Sanitaria del Hospital Cl\u00ednico San Carlos (IsISSC)  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pedro","family":"Arriola\u2010Villalobos","sequence":"additional","affiliation":[{"name":"Servicio de Oftalmolog\u00eda, Departamento de Inmunolog\u00eda, Oftalmolog\u00eda y ORL, Hospital Cl\u00ednico San Carlos, Facultad de Medicina, Universidad Complutense de Madrid Instituto de Investigaci\u00f3n Sanitaria del Hospital Cl\u00ednico San Carlos (IdISSC)  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carla M.","family":"Rico\u2010Luna","sequence":"additional","affiliation":[{"name":"Department of Microbiology Instituto de Investigaci\u00f3n Sanitaria del Hospital Cl\u00ednico San Carlos (IsISSC)  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo","family":"Cui\u00f1a\u2010Sardi\u00f1a","sequence":"additional","affiliation":[{"name":"Servicio de Oftalmolog\u00eda, Hospital Cl\u00ednico San Carlos Instituto de Investigaci\u00f3n Sanitaria del Hospital Cl\u00ednico San Carlos (IdISSC)  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberto","family":"Delgado\u2010Iribarren","sequence":"additional","affiliation":[{"name":"Department of Microbiology Instituto de Investigaci\u00f3n Sanitaria del Hospital Cl\u00ednico San Carlos (IsISSC)  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juli\u00e1n","family":"Garc\u00eda\u2010Feijo\u00f3","sequence":"additional","affiliation":[{"name":"Servicio de Oftalmolog\u00eda, Departamento de Inmunolog\u00eda, Oftalmolog\u00eda y ORL, Hospital Cl\u00ednico San Carlos, Facultad de Medicina, Universidad Complutense de Madrid Instituto de Investigaci\u00f3n Sanitaria del Hospital Cl\u00ednico San Carlos (IdISSC)  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,7,6]]},"reference":[{"volume-title":"Pneumonia of unknown cause\u2014China","year":"2020","author":"World Health Organization","key":"e_1_2_6_2_1"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaophthalmol.2020.1291"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30183-5"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.25725"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjophthalmol-2020-316304"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.genrep.2020.100682"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.idcr.2020.e00774"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2002032"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.25938"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2020.04.028"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00417-020-04695-8"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-020-0331-4"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2020.03.026"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.7326\/M20-1176"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/aos.14472"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.26219","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.26219","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jmv.26219","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.26219","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,3]],"date-time":"2023-09-03T09:54:38Z","timestamp":1693734878000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.26219"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,6]]},"references-count":15,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,1]]}},"alternative-id":["10.1002\/jmv.26219"],"URL":"https:\/\/doi.org\/10.1002\/jmv.26219","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"type":"print","value":"0146-6615"},{"type":"electronic","value":"1096-9071"}],"subject":[],"published":{"date-parts":[[2020,7,6]]},"assertion":[{"value":"2020-06-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-06-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-07-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}