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DEN outbreaks associated with multiple dengue virus (DV) serotypes have been regularly reported in different parts of India. This study was done during DEN outbreaks in 2015 to 2016 in UP and Bihar where DEN\u20102 was found as the only prevalent serotype. DV\u20102 was the only serotype amplified in serotype\u2010specific reverse\u2010transcription polymerase chain reaction from sera of 210 (65.21%) out of 322 DV NS1 antigen\u2010positive patients. Further genetic analysis based on full\u2010length envelope (E) protein sequence derived from patient's sera as well as DV isolate showed the circulation of lineages I and III of DV\u20102 cosmopolitan genotype during 2015 and lineage II during 2016. Finally, the phylogenetic analysis using the E gene sequence revealed that these DV\u20102 strains have a close genetic relationship with the recently reported DV\u20102 genotypes from DEN outbreaks reported from different parts of north India. These results showed the circulation of cosmopolitan genotype of DV\u20102 in eastern Uttar Pradesh and western Bihar, India. The genetic database generated on circulating DV strains in this study will be useful as reference for disease surveillance and strengthening laboratory diagnosis protocols.<\/jats:p>","DOI":"10.1002\/jmv.26239","type":"journal-article","created":{"date-parts":[[2020,7,7]],"date-time":"2020-07-07T14:58:10Z","timestamp":1594133890000},"page":"3322-3329","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Genetic characterization of dengue virus serotype 2 isolated from dengue fever outbreaks in eastern Uttar Pradesh and western Bihar, India"],"prefix":"10.1002","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4300-9956","authenticated-orcid":false,"given":"Hirawati","family":"Deval","sequence":"first","affiliation":[{"name":"Molecular Virology National Institute of Virology (Gorakhpur Unit) Gorakhpur Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1517-5757","authenticated-orcid":false,"given":"Sthita P.","family":"Behera","sequence":"additional","affiliation":[{"name":"Molecular Virology National Institute of Virology (Gorakhpur Unit) Gorakhpur Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Awdhesh","family":"Agrawal","sequence":"additional","affiliation":[{"name":"Division of Pathology Gorakhnath Hospital Gorakhpur Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rajeev","family":"Singh","sequence":"additional","affiliation":[{"name":"Molecular Virology National Institute of Virology (Gorakhpur Unit) Gorakhpur Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brijranjan","family":"Misra","sequence":"additional","affiliation":[{"name":"Molecular Virology National Institute of Virology (Gorakhpur Unit) Gorakhpur Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanka","family":"Janardhan","sequence":"additional","affiliation":[{"name":"Molecular Virology National Institute of Virology (Gorakhpur Unit) Gorakhpur Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gajanan","family":"Patil","sequence":"additional","affiliation":[{"name":"Molecular Virology National Institute of Virology (Gorakhpur Unit) Gorakhpur Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kamlesh","family":"Sah","sequence":"additional","affiliation":[{"name":"Molecular Virology National Institute of Virology (Gorakhpur Unit) Gorakhpur Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Niraj","family":"Kumar","sequence":"additional","affiliation":[{"name":"Molecular Virology National Institute of Virology (Gorakhpur Unit) Gorakhpur Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ravishankar","family":"Singh","sequence":"additional","affiliation":[{"name":"Molecular Virology National Institute of Virology (Gorakhpur Unit) Gorakhpur Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1958-6270","authenticated-orcid":false,"given":"Vijay P.","family":"Bondre","sequence":"additional","affiliation":[{"name":"Encephalitis Group ICMR\u2013National Institute of Virology, Microbial Containment Complex Pune Maharashtra India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,7,17]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"Scientific Working Group on Dengue & UNDP\/World Bank\/WHO Special Programme for Research and Training in Tropical Diseases. 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