{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T20:21:16Z","timestamp":1790022076093,"version":"4.0.1"},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,7,14]],"date-time":"2020-07-14T00:00:00Z","timestamp":1594684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The initial cases of severe acute respiratory syndrome coronavirus\u20102 (SARS\u2010CoV\u20102) occurred in Wuhan, China, in December 2019 and swept the world by 23 June 2020 with 8\u2009993\u2009659 active cases, 469\u2009587 deaths across 216 countries, areas or territories. This strongly implies global transmission occurred before the lockdown of China. However, the initial source's transmission routes of SARS\u2010CoV\u20102 remain obscure and controversial. Research data suggest bat (RaTG13) and pangolin carried CoV were the proximal source of SARS\u2010CoV\u20102. In this study, we used systematic phylogenetic analysis of\n                    <jats:italic>Coronavirinae<\/jats:italic>\n                    subfamily along with wild type human SARS\u2010CoV, MERS\u2010CoV, and SARS\u2010CoV\u20102 strains. The key residues of the receptor\u2010binding domain (RBD) and O\u2010linked glycan were compared. SARS\u2010CoV\u20102 strains were clustered with RaTG13 (97.41% identity), Pangolin\u2010CoV (92.22% identity) and Bat\u2010SL\u2010CoV (80.36% identity), forms a new clade\u20102 in lineage B of beta\u2010CoV. The alignments of RBD contact residues to ACE2 justified? Those SARS\u2010CoV\u20102 strains sequences were 100% identical by each other, significantly varied in RaTG13 and pangolin\u2010CoV. SARS\u2010CoV\u20102 has a polybasic cleavage site with an inserted sequence of PRRA compared to RaTG13 and only PRR to pangolin. Only serine (Ser) in pangolin and both threonine (Thr) and serine (Ser) O\u2010linked glycans were seen in RaTG13, suggesting that a detailed study needed in pangolin (\n                    <jats:italic>Manis javanica<\/jats:italic>\n                    ) and bat (\n                    <jats:italic>Rhinolophus affinis<\/jats:italic>\n                    ) related CoV.\n                  <\/jats:p>","DOI":"10.1002\/jmv.26261","type":"journal-article","created":{"date-parts":[[2020,7,7]],"date-time":"2020-07-07T10:53:56Z","timestamp":1594119236000},"page":"499-505","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["An update on the origin of SARS\u2010CoV\u20102: Despite closest identity, bat (RaTG13) and pangolin derived coronaviruses varied in the critical binding site and O\u2010linked glycan residues"],"prefix":"10.1002","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6466-308X","authenticated-orcid":false,"given":"Jeevan","family":"Malaiyan","sequence":"first","affiliation":[{"name":"Division of Virology, Department of Microbiology, Sri Muthukumaran Medical College Hospital and Research Institute Affiliated to The Tamil Nadu Dr. M.G.R. Medical University  Chikkarayapuram Chennai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6247-1156","authenticated-orcid":false,"given":"Suresh","family":"Arumugam","sequence":"additional","affiliation":[{"name":"Central Research Facility, Meenakshi Medical College Hospital and Research Institute Meenakshi Academy of Higher Education and Research  Kancheepuram Tamilnadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamalraj","family":"Mohan","sequence":"additional","affiliation":[{"name":"Division of Virology, Department of Microbiology, Sri Muthukumaran Medical College Hospital and Research Institute Affiliated to The Tamil Nadu Dr. M.G.R. Medical University  Chikkarayapuram Chennai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gokul","family":"Gomathi Radhakrishnan","sequence":"additional","affiliation":[{"name":"Department of General Medicine, Sri Muthukumaran Medical College Hospital and Research Institute Affiliated to The Tamil Nadu Dr. M.G.R. Medical University  Chikkarayapuram Chennai India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,7,14]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30251-8"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm9030637"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.03.035"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40779-020-00240-0"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12098-020-03263-6"},{"key":"e_1_2_9_7_1","unstructured":"WHO Coronavirus Disease (COVID\u201019) Dashboard.https:\/\/covid19.who.int. 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