{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T13:25:37Z","timestamp":1783689937426,"version":"3.55.0"},"reference-count":20,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,10,7]],"date-time":"2020-10-07T00:00:00Z","timestamp":1602028800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Hepatitis E virus (HEV) is a major causative agent of acute viral hepatitis in many regions of the world including Africa. In Cameroon, there is no published molecular study on HEV in humans. However, based on serological assays, the first outbreak of HEV was detected in North\u2010Cameroon. The objective of this study was to determine the molecular characterization of HEV that circulated during this period. A retrospective study design was used to select serum samples among those collected during the outbreak period. immunoglobulin M positive samples available in sufficient volumes to amplify HEV RNA were selected. RNA was extracted and then amplified by a real\u2010time reverse transcription polymerase chain reaction (real time RT\u2010PCR) assay, followed by a nested reverse transcription polymerase chain reaction (nested RT\u2010PCR) assay for sequencing and phylogenetic analysis. Overall, 24 samples were selected and HEV RNA was amplified by real\u2010time RT\u2010PCR in 20 samples. Amongst these, 12 samples were positive for HEV RNA by nested RT\u2010PCR and yielded good sequencing products. Phylogenetic analysis showed that 10 samples clustered with HEV genotype 1 (subtype 1e) and two samples clustered with HEV genotype 3 (subtype 3f). This study fills the gap of knowledge on the molecular epidemiology of HEV in Cameroon and confirms the first report of the hepatitis E outbreak in North\u2010Cameroon.<\/jats:p>","DOI":"10.1002\/jmv.26277","type":"journal-article","created":{"date-parts":[[2020,7,8]],"date-time":"2020-07-08T14:36:18Z","timestamp":1594218978000},"page":"4018-4022","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["First molecular characterization of the hepatitis E virus in humans in Cameroon: Confirmation of the HEV outbreak in Touboro, North\u2010Cameroon"],"prefix":"10.1002","volume":"93","author":[{"given":"Abdou Fatawou","family":"Modiyinji","sequence":"first","affiliation":[{"name":"Department of Virology Centre Pasteur of Cameroon Yaound\u00e9 Cameroon"},{"name":"Department of Animals Biology and Physiology Faculty of Sciences University of Yaound\u00e9 I Yaound\u00e9 Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Rivero\u2010Juarez","sequence":"additional","affiliation":[{"name":"Unidad de Enfermedades Infecciosas Hospital Universitario Reina Sof\u00eda de C\u00f3rdoba Instituto Maimonides de Investigaci\u00f3n Biom\u00e9dica de C\u00f3rdoba (IMIBIC) Universidad de C\u00f3rdoba C\u00f3rdoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro","family":"Lopez\u2010Lopez","sequence":"additional","affiliation":[{"name":"Unidad de Enfermedades Infecciosas Hospital Universitario Reina Sof\u00eda de C\u00f3rdoba Instituto Maimonides de Investigaci\u00f3n Biom\u00e9dica de C\u00f3rdoba (IMIBIC) Universidad de C\u00f3rdoba C\u00f3rdoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie Amougou","family":"Atsama","sequence":"additional","affiliation":[{"name":"Department of Virology Centre Pasteur of Cameroon Yaound\u00e9 Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chavely Gwladys","family":"Monamele","sequence":"additional","affiliation":[{"name":"Department of Virology Centre Pasteur of Cameroon Yaound\u00e9 Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moise","family":"Nola","sequence":"additional","affiliation":[{"name":"Department of Animals Biology and Physiology Faculty of Sciences University of Yaound\u00e9 I Yaound\u00e9 Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Rivero","sequence":"additional","affiliation":[{"name":"Unidad de Enfermedades Infecciosas Hospital Universitario Reina Sof\u00eda de C\u00f3rdoba Instituto Maimonides de Investigaci\u00f3n Biom\u00e9dica de C\u00f3rdoba (IMIBIC) Universidad de C\u00f3rdoba C\u00f3rdoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3112-6370","authenticated-orcid":false,"given":"Richard","family":"Njouom","sequence":"additional","affiliation":[{"name":"Department of Virology Centre Pasteur of Cameroon Yaound\u00e9 Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,10,7]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"WHOHepatitis E Fact Sheet. 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