{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T01:23:09Z","timestamp":1778635389087,"version":"3.51.4"},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,8,13]],"date-time":"2020-08-13T00:00:00Z","timestamp":1597276800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Serology testing of severe acute respiratory syndrome coronavirus 2 (SARS\u2010CoV\u20102) is increasingly being used during the current pandemic of coronavirus disease 2019 (COVID\u201019), although its clinical and epidemiologic utilities are still debatable. Characterizing these assays provides scientific basis to best use them. The current study assessed one chemiluminescent assay (Abbott COVID\u20102 IgG) and two lateral flow assays (STANDARD Q [SQ] IgM\/IgG Duo and Wondfo total antibody test) using 113 blood samples from 71 PCR\u2010confirmed COVID\u201019 hospitalized patients, 119 samples with potential cross\u2010reactions, and 1068 negative controls including 942 pre\u2010pandemic samples. SARS\u2010CoV\u20102 IgM antibodies became detectable 3\u20104 days post\u2010symptom onset using SQ IgM test and IgG antibodies were first detected 5\u20106 days post\u2010onset using SQ IgG. Abbott IgG and Wondfo Total were able to detect antibodies 7 to 8 days post\u2010onset. After 14 days post\u2010symptom onset, the SQ IgG, Abbott IgG and Wondfo Total tests were able to detect antibodies from 100% of the PCR\u2010confirmed patients in this series; 87.5% sensitivity for SQ IgM. Overall agreement was 88.5% between SQ IgM\/IgG and Wondfo Total and 94.6% between SQ IgG and Abbott IgG. No cross\u2010reaction due to recent sera with three of the endemic coronaviruses was observed. Viral hepatitis and autoimmune samples were the main source of limited cross\u2010reactions. The specificities were 100% for SQ IgG and Wondfo Total, 99.62% for Abbott IgG, and 98.87% for SQ IgM. These findings demonstrated high sensitivity and specificity of appropriately validated SARS\u2010CoV\u20102 serologic assays with implications for clinical use and epidemiological seroprevalence studies.<\/jats:p>","DOI":"10.1002\/jmv.26341","type":"journal-article","created":{"date-parts":[[2020,7,25]],"date-time":"2020-07-25T09:09:58Z","timestamp":1595668198000},"page":"916-923","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Validation and performance comparison of three SARS\u2010CoV\u20102 antibody assays"],"prefix":"10.1002","volume":"93","author":[{"given":"Kimberly J.","family":"Paiva","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Warren Alpert Medical School of Brown University  Providence Rhode Island"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricky D.","family":"Grisson","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Warren Alpert Medical School of Brown University  Providence Rhode Island"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philip A.","family":"Chan","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases Warren Alpert Medical School of Brown University  Providence Rhode Island"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard C.","family":"Huard","sequence":"additional","affiliation":[{"name":"Rhode Island State Laboratory Rhode Island Department of Health  Providence Rhode Island"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela M.","family":"Caliendo","sequence":"additional","affiliation":[{"name":"Department of Medicine Warren Alpert Medical School of Brown University  Providence Rhode Island"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John R.","family":"Lonks","sequence":"additional","affiliation":[{"name":"Department of Medicine Warren Alpert Medical School of Brown University  Providence Rhode Island"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ewa","family":"King","sequence":"additional","affiliation":[{"name":"Rhode Island State Laboratory Rhode Island Department of Health  Providence Rhode Island"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric W.","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Warren Alpert Medical School of Brown University  Providence Rhode Island"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diane L.","family":"Pytel\u2010Parenteau","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Warren Alpert Medical School of Brown University  Providence Rhode Island"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ga H.","family":"Nam","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Warren Alpert Medical School of Brown University  Providence Rhode Island"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evgeny","family":"Yakirevich","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Warren Alpert Medical School of Brown University  Providence Rhode Island"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2870-5793","authenticated-orcid":false,"given":"Shaolei","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Warren Alpert Medical School of Brown University  Providence Rhode Island"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,8,13]]},"reference":[{"key":"e_1_2_9_2_1","unstructured":"COVID\u201019 Dashboard by the Center for Systems Science and Engineering (CSSE) at Johns Hopkins University (JHU).https:\/\/coronavirus.jhu.edu\/map.html. Accessed 30 June 2020."},{"issue":"7798","key":"e_1_2_9_3_1","first-page":"265","article-title":"A new coronavirus associated with human respiratory disease in China [published correction appears in Nature. 2020;580(7803):E7]","volume":"579","author":"Wahba L","year":"2020","journal-title":"Nature"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/14737159.2020.1757437"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa310"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa344"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2020.1773324"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2020.03.051"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiaa273"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00461-20"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00941-20"},{"key":"e_1_2_9_12_1","unstructured":"Wondfo.\u00a0SARS\u2010CoV\u20102 Antibody Test (Lateral Flow Meth\u00a0od) package insert. Wondfo Guangzhou China;\u00a02020."},{"key":"e_1_2_9_13_1","unstructured":"BiosensorSD.\u00a0STANDARDTM Q COVID\u201019 IgM\/IgG Duo Test package insert. Gyeonggi\u2010do Korea;2020."},{"key":"e_1_2_9_14_1","unstructured":"Abbott.\u00a0ARCHITECT SARS\u2010CoV\u20102 IgG package insert. Abbott Diagnostics Lake Forest IL;2020."},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00124-08"},{"key":"e_1_2_9_16_1","article-title":"Clinical performance of two SARS\u2010CoV\u20102 serologic assays [published online ahead of print May 13, 2020]","author":"Tang MS","year":"2020","journal-title":"Clin Chem"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.25715"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/1469-0691.12525"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268800048019"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00941-20"},{"issue":"3","key":"e_1_2_9_21_1","first-page":"253","article-title":"Unexpected positive prospective crossmatches in organ transplant","volume":"15","author":"Desoutter J","year":"2017","journal-title":"Exp Clin Transplant"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2018.00841"},{"key":"e_1_2_9_23_1","doi-asserted-by":"crossref","unstructured":"WhitmanJD HiattJ MoweryCT et al. Test performance evaluation of SARS\u2010CoV\u20102 serological assays [published online ahead of print May 17 2020]. Preprint.medRxiv. 2020.https:\/\/doi.org\/10.1101\/2020.04.25.20074856","DOI":"10.1101\/2020.04.25.20074856"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.2196\/18606"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1080\/07853890.2020.1763449"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa523"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2020.1756699"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.26341","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.26341","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jmv.26341","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.26341","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T10:26:18Z","timestamp":1693563978000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.26341"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,13]]},"references-count":26,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2021,2]]}},"alternative-id":["10.1002\/jmv.26341"],"URL":"https:\/\/doi.org\/10.1002\/jmv.26341","archive":["Portico"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.05.29.124776","asserted-by":"object"}]},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8,13]]},"assertion":[{"value":"2020-07-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-07-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}