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Clinical, epidemiological data and nasopharyngeal swabs were prospectively collected from children aged less than 5 years presenting with acute respiratory infections from October 2016 to September 2018. Real\u2010time polymerase chain reaction for 12 respiratory viruses, and sequencing, phylogenetic, and amino acid analyses of the RSV G gene\/protein were performed. Of the 875 children examined, 645 (73.7%) were positive for at least one viral respiratory pathogen. RSV was the most commonly detected virus (26.2%), followed by rhinoviruses (15%), influenza A (H3N2) (9.7%), adenoviruses (9%), bocaviruses (7.2%), human metapneumovirus (6.1%), parainfluenza viruses 1\/2\/3 (5.8%), influenza type B (5.5%), and A(H1N1)pdm09 (3.4%). The detection rate for RSV varied across two winter seasons (36.7% vs 20.3%). RSV\u2010B cases outnumbered those of the RSV\u2010A throughout the study period. RSV was the most common virus detected in patients with bronchiolitis (45.1%) and pneumonia (24%). Phylogenetic analysis indicated that all the sequenced RSV\u2010A strains belonged to the ON1 genotype and the RSV\u2010B strains were classified as BA9 genotype. Amino acid substitutions at 15 and 22 positions of the HVR\u20102 were identified compared with the ON1 and BA prototype strains,\u00a0respectively. This study revealed the leading role of RSV as a causative agent of serious respiratory illnesses in early childhood, year\u2010on\u2010year fluctuations in RSV incidence, the dominance of RSV\u2010B, and relatively low genetic diversity in the circulating RSV strains.<\/jats:p>","DOI":"10.1002\/jmv.26415","type":"journal-article","created":{"date-parts":[[2020,8,12]],"date-time":"2020-08-12T02:36:35Z","timestamp":1597199795000},"page":"3401-3411","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Predominance of ON1 and BA9 genotypes of respiratory syncytial virus (RSV) in Bulgaria, 2016\u20102018"],"prefix":"10.1002","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3188-2283","authenticated-orcid":false,"given":"Neli","family":"Korsun","sequence":"first","affiliation":[{"name":"Department of Virology National Center of Infectious and Parasitic Diseases Sofia Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svetla","family":"Angelova","sequence":"additional","affiliation":[{"name":"Department of Virology National Center of Infectious and Parasitic Diseases Sofia Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivelina","family":"Trifonova","sequence":"additional","affiliation":[{"name":"Department of Virology National Center of Infectious and Parasitic Diseases Sofia Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Voleva","sequence":"additional","affiliation":[{"name":"Department of Virology National Center of Infectious and Parasitic Diseases Sofia Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iliana","family":"Grigorova","sequence":"additional","affiliation":[{"name":"Department of Virology National Center of Infectious and Parasitic Diseases Sofia Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iren","family":"Tzotcheva","sequence":"additional","affiliation":[{"name":"Pediatric Clinic, University Hospital Alexandrovska Medical University Sofia Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sirma","family":"Mileva","sequence":"additional","affiliation":[{"name":"Pediatric Clinic, University Hospital Alexandrovska Medical University Sofia Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivaylo","family":"Alexiev","sequence":"additional","affiliation":[{"name":"Department of Virology National Center of Infectious and Parasitic Diseases Sofia Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Penka","family":"Perenovska","sequence":"additional","affiliation":[{"name":"Pediatric Clinic, University Hospital Alexandrovska Medical University Sofia Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,10,30]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.2.539-542.1998"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(17)30938-8"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx932"},{"key":"e_1_2_8_5_1","first-page":"1086","volume-title":"Fields Virology","author":"Collins PL","year":"2013"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.2217\/fmb-2018-0261"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2019.104200"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2019.10.009"},{"key":"e_1_2_8_9_1","first-page":"286","volume-title":"Fields Virology","author":"Holmes EC","year":"2013"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-66-10-2111"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.80.2.975-984.2006"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2014.09.030"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep14268"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.24640"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02636-10"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02270-10"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00889-06"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00339-07"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02286-08"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.14.9157-9167.2005"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-82-9-2117"},{"key":"e_1_2_8_22_1","unstructured":"GuptaR JungE BrunakS. 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