{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T20:02:36Z","timestamp":1759089756350,"version":"3.37.3"},"reference-count":28,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,10,14]],"date-time":"2020-10-14T00:00:00Z","timestamp":1602633600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Yellow fever is an acute infectious disease that is common in Africa and South America and causes thousands of deaths annually. However, there are very few studies on yellow fever virus (YFV) antigen detection kits. As a detection target, the nonstructural protein 1 (NS1) has been successfully used in the early diagnosis of dengue virus (a member of the Flaviviridae family) infection. In this study, we used monoclonal antibody technology to prepare anti\u2010YFV NS1 monoclonal antibodies (MAbs) and identified their immunological properties. Next, we used two mouse MAbs that can recognize different epitopes of YFV NS1 as capture and detection antibodies to establish a YFV NS1 antigen\u2010capture enzyme\u2010linked immunosorbent assay (ELISA). The antigen\u2010capture ELISA displayed exclusive specificity to YFV without cross\u2010reaction with other related members of the flavivirus family, including the dengue virus, West Nile virus, Japanese encephalitis virus. Additionally, the detection sensitivity towards the YFV culture supernatant was 103 TCID50\/mL and the detection positivity rate was 95% compared with reverse transcription\u2010polymerase chain reaction. In conclusion, this newly developed NS1 antigen\u2010capture ELISA with high sensitivity and specificity could be used as an efficient method for the early diagnosis of YFV infection in animals or humans.<\/jats:p>","DOI":"10.1002\/jmv.26455","type":"journal-article","created":{"date-parts":[[2020,8,26]],"date-time":"2020-08-26T02:49:26Z","timestamp":1598410166000},"page":"3374-3382","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Preparation and application of yellow fever virus NS1 protein\u2010specific monoclonal antibodies"],"prefix":"10.1002","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3616-917X","authenticated-orcid":false,"given":"Duoduo","family":"Liu","sequence":"first","affiliation":[{"name":"Medicine Laboratory Nanfang Hospital of Southern Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongmiao","family":"Chen","sequence":"additional","affiliation":[{"name":"Medicine Laboratory Nanfang Hospital of Southern Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tingting","family":"Zhang","sequence":"additional","affiliation":[{"name":"Medicine Laboratory Nanfang Hospital of Southern Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nan","family":"Yu","sequence":"additional","affiliation":[{"name":"Division of Laboratory Medicine and Laboratory of Emerging Infectious Diseases Zhujiang Hospital of Southern Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruiwen","family":"Ren","sequence":"additional","affiliation":[{"name":"Guangdong Arbovirus Diseases Emergency Technology Research Center Center for Disease Prevention and Control of Southern Theatre Command of PLA Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Chen","sequence":"additional","affiliation":[{"name":"Guangdong Arbovirus Diseases Emergency Technology Research Center Center for Disease Prevention and Control of Southern Theatre Command of PLA Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Congrong","family":"Wang","sequence":"additional","affiliation":[{"name":"Medicine Laboratory Nanfang Hospital of Southern Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,10,14]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cll.2010.01.001"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2016.05.001"},{"issue":"32","key":"e_1_2_9_4_1","first-page":"442","article-title":"Yellow fever in Africa and the Americas, 2016","volume":"92","author":"Hamrick PN","year":"2017","journal-title":"Wkly Epidemiol Rec"},{"issue":"7","key":"e_1_2_9_5_1","article-title":"A fatal yellow fever virus infection in China: description and lessons","volume":"5","author":"Chen Z","year":"2016","journal-title":"Emerging Microbes Infect"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00827-18"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00209-16"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2016.04.025"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-8-261"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-16231-6"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3969\/j.issn.1001-9448.2006.03.003"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3969\/j.issn.1002-2694.2007.12.003"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.3969\/j.issn.1001-9448.2007.11.002"},{"issue":"07","key":"e_1_2_9_14_1","first-page":"777","article-title":"Production and characterization of the monoclonal antibodies against nonstructural 1 protein of DENV\u20104. 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