{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T22:50:36Z","timestamp":1771627836739,"version":"3.50.1"},"reference-count":24,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,9,29]],"date-time":"2020-09-29T00:00:00Z","timestamp":1601337600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Viral meningitis is the most common type of meningitis. Worldwide, nonpolio enteroviruses (NPEVs) account for 23%\u201360% of all cases of viral meningitis. We aimed to detect NPEV among aseptic meningitis cases using reverse transcription\u2010polymerase chain reaction\u00a0(RT\u2010PCR) and evaluate molecular testing versus clinical and laboratory parameters.<\/jats:p><\/jats:sec><jats:sec><jats:title>Patients and Methods<\/jats:title><jats:p>A 2\u2010year prospective study was conducted for all clinically suspected meningitis patients, who underwent lumbar puncture in Alshatby University and Alexandria Fever Hospitals. Clinical manifestations were reviewed; cytological, microbiological, and biochemical examinations were done. One\u2010step RT\u2010PCR for NPEV was introduced to a routine workflow using Pan\u2010Enterovirus primers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Out of 2519 patients, 994 (40%) patients were found to have positive cerebrospinal fluid findings, out of which 716 (72%) patients had positive findings of aseptic meningitis. Ninety\u2010four samples were randomly selected and divided across four age groups: neonates, infants, children, and adults. The significant difference was found among adult patients regarding fever, vomiting, headache, signs of meningeal irritation, cranial nerve affection, and focal neurological deficits (<jats:italic>p<\/jats:italic>\u2009\u2264\u2009.05). Seven cases (7.4%) were found to be NPEV positive by RT\u2010PCR. Positive NPEV PCR samples were shown to be statistically significant among neonates (<jats:italic>p<\/jats:italic>\u2009\u2264\u2009.05). The statistical significance was found among the NPEV group regarding the length of hospital stay and duration of IV antibiotic intake\u00a0while no statistical significance was found with any clinical or laboratory findings.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>RT\u2010PCR was reliable to identify NPEV\u00a0while clinical and laboratory findings were inconclusive. NPEV showed low incidence and slight seasonal variation which rings the bell to investigate other causes of viral meningitis throughout the year.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.26480","type":"journal-article","created":{"date-parts":[[2020,9,3]],"date-time":"2020-09-03T14:53:33Z","timestamp":1599144813000},"page":"3389-3396","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Clinical and laboratory detection of nonpolio enteroviruses among different age groups of aseptic meningitis patients in Alexandria, Egypt"],"prefix":"10.1002","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6750-3719","authenticated-orcid":false,"given":"May","family":"Raouf","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology and Immunology, Faculty of Medicine Alexandria University Alexandria Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ola 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