{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T10:06:02Z","timestamp":1770458762547,"version":"3.49.0"},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,9,28]],"date-time":"2020-09-28T00:00:00Z","timestamp":1601251200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004686","name":"Deanship of Scientific Research, King Faisal University","doi-asserted-by":"publisher","award":["1811016"],"award-info":[{"award-number":["1811016"]}],"id":[{"id":"10.13039\/501100004686","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>The papain\u2010like protease (PL<jats:sup>pro<\/jats:sup>) is an important enzyme for coronavirus polyprotein processing, as well as for virus\u2010host immune suppression. Previous studies reveal that a molecular analysis of PL<jats:sup>pro<\/jats:sup> indicates the catalytic activity of viral PL<jats:sup>pro<\/jats:sup> and its interactions with ubiquitin. By using sequence comparisons, molecular models, and protein\u2013protein interaction maps, PL<jats:sup>pro<\/jats:sup> was compared in the three recorded fatal CoV epidemics, which involved severe acute respiratory syndrome coronavirus 2 (SARS\u2010CoV\u20102), severe acute respiratory syndrome CoV (SARS\u2010CoV), and Middle East respiratory syndrome coronavirus (MERS\u2010CoV). The pairwise sequence comparison of SARS\u2010CoV\u20102 PL<jats:sup>pro<\/jats:sup> indicated similarity percentages of 82.59% and 30.06% with SARS\u2010CoV PL<jats:sup>pro<\/jats:sup> and MERS\u2010CoV PL<jats:sup>pro<\/jats:sup>, respectively. In comparison with SARS\u2010CoV PL<jats:sup>pro<\/jats:sup>, in SARS\u2010CoV\u20102, the PL<jats:sup>pro<\/jats:sup> had a conserved catalytic triad of C111, H278, and D293, with a slightly lower number of polar interface residues and of hydrogen bonds, a higher number of buried interface sizes, and a lower number of residues that interact with ubiquitin and PL<jats:sup>pro<\/jats:sup>. These features might contribute to a similar or slightly lower level of deubiquitinating activity in SARS\u2010CoV\u20102 PLpro. It was, however, a much higher level compared to MERS\u2010CoV, which contained amino acid mutations and a low number of polar interfaces. SARS\u2010CoV\u20102 PL<jats:sup>pro<\/jats:sup> and SARS\u2010CoV PL<jats:sup>pro<\/jats:sup> showed almost the same catalytic site profiles, interface area compositions and polarities, suggesting a general similarity in deubiquitination activity. Compared with MERS\u2010CoV, SARS\u2010CoV\u20102 had a higher potential for binding interactions with ubiquitin. These estimated parameters contribute to the knowledge gap in understanding how the new virus interacts with the immune system.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.26497","type":"journal-article","created":{"date-parts":[[2020,9,14]],"date-time":"2020-09-14T07:45:24Z","timestamp":1600069524000},"page":"1581-1588","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["The emerging SARS\u2010CoV\u20102 papain\u2010like protease: Its relationship with recent coronavirus epidemics"],"prefix":"10.1002","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3668-5147","authenticated-orcid":false,"given":"Mahmoud","family":"Kandeel","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences, College of Veterinary Medicine King Faisal University  Al\u2010Ahsa Saudi Arabia"},{"name":"Department of Pharmacology, Faculty of Veterinary Medicine Kafrelshikh University  Kafrelshikh Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukio","family":"Kitade","sequence":"additional","affiliation":[{"name":"Department of Applied Chemistry, Faculty of Engineering Aichi Institute of Technology  Toyota Aichi Japan"},{"name":"Department of Chemistry and Biomolecular Sciences, Faculty of Engineering Gifu University  Gifu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2145-2714","authenticated-orcid":false,"given":"Mahmoud","family":"Fayez","sequence":"additional","affiliation":[{"name":"Ministry of Agriculture Al\u2010Ahsa Veterinary Diagnostic Laboratory  Saudi Arabia"},{"name":"Veterinary Serum and Vaccine Research institute, Ministry of Agriculture  Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katharigatta N.","family":"Venugopala","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, College of Clinical Pharmacy King Faisal University  Al\u2010Ahsa Saudi Arabia"},{"name":"Department of Biotechnology and Food Technology Durban University of Technology  Durban South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdelazim","family":"Ibrahim","sequence":"additional","affiliation":[{"name":"Department of Pathology King Faisal University  Al\u2010Ahsa Saudi Arabia"},{"name":"Department of Pathology, Faculty of Veterinary Medicine Suez Canal University  Ismailia Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,9,28]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11427-020-1637-5"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)13077-2"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.80.9.4211-4219.2006"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1211721"},{"key":"e_1_2_7_6_1","doi-asserted-by":"crossref","unstructured":"Group WM\u2010CR.State of knowledge and data gaps of Middle East respiratory syndrome coronavirus (MERS\u2010CoV) in humans.PLOS Currents Outbreaks.2013.","DOI":"10.1016\/S1773-035X(13)72154-3"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(15)60859-5"},{"key":"e_1_2_7_8_1","first-page":"25","article-title":"Preliminary epidemiologic assessment of MERS\u2010CoV outbreak in South Korea","volume":"20","author":"Cowling BJ","year":"2015","journal-title":"May\u2013June 2015. 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