{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T09:34:04Z","timestamp":1758706444478,"version":"3.40.5"},"reference-count":41,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,10,14]],"date-time":"2020-10-14T00:00:00Z","timestamp":1602633600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004748","name":"Mashhad University of Medical Sciences","doi-asserted-by":"publisher","award":["86645"],"award-info":[{"award-number":["86645"]}],"id":[{"id":"10.13039\/501100004748","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Adult T\u2010cell leukemia (ATL) is a life\u2010threatening malignant neoplasm of CD4<jats:sup>+<\/jats:sup> T cells resulted from human T\u2010cell leukemia virus type I (HTLV\u2010I). Tax1 protein of HTLV\u2010I can induce malignant proliferation of T\u2010cells by\u00a0modulating the expression of growth factors such as platelet\u2010derived growth factor (PDGF). Here, we aimed to investigate the proviral load (PVL) of HTLV\u2010I in ATL\u00a0and also to evaluate the mRNA expression of B chain of PDGF and PDGF\u2010\u03b2 receptors in ATL patients and HTLV\u2010I\u2010infected healthy carriers. To this end, peripheral blood mononuclear cells (PBMCs) were isolated by using Ficoll\u2010Histophaque density centrifugation. The mean of HTLV\u2010I PVL in ATL patients (42,759\u2009\u00b1\u200915,737 copies\/10<jats:sup>4<\/jats:sup> cells [95% CI, 9557\u201075962]) was significantly (<jats:italic>p<\/jats:italic>\u2009=\u2009.01) higher than that in healthy carriers (650\u2009\u00b1\u2009107 copies\/10<jats:sup>4<\/jats:sup> cells [95% CI, 422\u2010879], respectively. The HTLV\u2010I PVL in ATL patients exhibited a significant correlation with PBMC count (<jats:italic>R<\/jats:italic>\u2009=\u2009.495, <jats:italic>p\u2009=\u2009<\/jats:italic>.001). The mRNA expression of Tax, B chain of PDGF, and PDGF\u2010\u03b2 receptor genes was significantly higher in healthy carriers than in patients with ATL. In conclusion, the expression of the canonical PDGF\u03b2 and its receptor, and their correlation with Tax expression cannot be a suitable indicator and\/or prognostic factor for progression of ATL in HTLV\u2010I carriers.<\/jats:p>","DOI":"10.1002\/jmv.26510","type":"journal-article","created":{"date-parts":[[2020,9,25]],"date-time":"2020-09-25T11:46:24Z","timestamp":1601034384000},"page":"3865-3870","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Evaluating mRNA expression of <i>tax<\/i>, B chain of PDGF\u00a0and PDGF\u2010\u03b2 receptors\u00a0as well as HTLV\u2010I proviral load in ATL patients and healthy carriers"],"prefix":"10.1002","volume":"93","author":[{"given":"Hadi","family":"Atabati","sequence":"first","affiliation":[{"name":"Immunology Research Centre, Division of Inflammation and Inflammatory Diseases Mashhad University of Medical Sciences Mashhad Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seyed\u2010Alireza","family":"Esmaeili","sequence":"additional","affiliation":[{"name":"Immunology Research Center Mashhad University of Medical Sciences Mashhad Iran"},{"name":"Immunology Department Faculty of Medicine, Mashhad University of Medical Sciences Mashhad Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abolghasem","family":"Allahyari","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Imam Reza Hospital, Mashhad University of Medical Sciences Mashhad Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abbas","family":"Shirdel","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Ghaem Hospital, Mashhad University of Medical Sciences Mashhad Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hossein","family":"Rahimi","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Ghaem Hospital, Mashhad University of Medical Sciences Mashhad Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seyed Abdolrahim","family":"Rezaee","sequence":"additional","affiliation":[{"name":"Immunology Research Centre, Division of Inflammation and Inflammatory Diseases Mashhad University of Medical Sciences Mashhad Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4376-1083","authenticated-orcid":false,"given":"Amir A.","family":"Momtazi\u2010Borojeni","sequence":"additional","affiliation":[{"name":"Halal Research Center of IRI FDA Tehran Iran"},{"name":"Department of Medical Biotechnology Faculty of Medicine, Mashhad University of Medical Sciences Mashhad Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Houshang","family":"Rafatpanah","sequence":"additional","affiliation":[{"name":"Immunology Research Centre, Division of Inflammation and Inflammatory Diseases Mashhad University 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