{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T07:09:15Z","timestamp":1792652955761,"version":"4.5.0"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,10,5]],"date-time":"2020-10-05T00:00:00Z","timestamp":1601856000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Coronavirus disease 2019 (COVID\u201019)\u00a0has become pandemic since March\u00a011, 2020. Thus, development and integration in clinics of fast and sensitive diagnostic tools are essential. The aim of the study is\u00a0a development and evaluation of a one\u2010step quantitative\u00a0reverse transcription\u2010polymerase chain reaction (RT\u2010qPCR) assay (COVID\u201019 Amp) for severe acute respiratory syndrome coronavirus 2 (SARS\u2010CoV\u20102) detection with an armored positive control and internal controls constructed from synthetic MS2\u2010phage\u2010based RNA particles. The COVID\u201019 Amp assay limit of detection was 10<jats:sup>3<\/jats:sup>\u2009copies\/ml, the analytical specificity was 100%. A total of 109 biological samples were examined using COVID\u201019 Amp and World Health Organization (WHO)\u2010based assay. Discordance in nine samples was observed (negative by the WHO\u2010based assay) and discordant samples were retested as positive according to the results obtained from the Vector\u2010PCRrv\u20102019\u2010nCoV\u2010RG assay. The developed COVID\u201019 Amp assay has high sensitivity and specificity, includes virus particles\u2010based controls, provides the direct definition of the SARS\u2010CoV\u20102 RdRp gene partial sequence, and is suitable for any hospital and laboratory equipped for RT\u2010qPCR.<\/jats:p>","DOI":"10.1002\/jmv.26540","type":"journal-article","created":{"date-parts":[[2020,9,23]],"date-time":"2020-09-23T16:33:46Z","timestamp":1600878826000},"page":"1694-1701","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["One\u2010step quantitative RT\u2010PCR assay with armored RNA controls for detection of SARS\u2010CoV\u20102"],"prefix":"10.1002","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4891-0439","authenticated-orcid":false,"given":"Ekaterina A.","family":"Goncharova","sequence":"first","affiliation":[{"name":"Department of Epidemiology Pasteur Institute, Federal Service on Consumers\u2019 Rights Protection and Human Well\u2010Being Surveillance Saint Petersburg Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir G.","family":"Dedkov","sequence":"additional","affiliation":[{"name":"Department of Epidemiology Pasteur Institute, Federal Service on Consumers\u2019 Rights Protection and Human Well\u2010Being Surveillance Saint Petersburg Russia"},{"name":"Martsinovsky Institute of Medical Parasitology, Tropical and Vector Borne Diseases Sechenov First Moscow State Medical University Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna S.","family":"Dolgova","sequence":"additional","affiliation":[{"name":"Department of Epidemiology Pasteur Institute, Federal Service on Consumers\u2019 Rights Protection and Human Well\u2010Being Surveillance Saint Petersburg Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4736-2183","authenticated-orcid":false,"given":"Ilia S.","family":"Kassirov","sequence":"additional","affiliation":[{"name":"Department of Epidemiology Pasteur Institute, Federal Service on Consumers\u2019 Rights Protection and Human Well\u2010Being Surveillance Saint Petersburg Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina V.","family":"Safonova","sequence":"additional","affiliation":[{"name":"Department of Particularly Dangerous Diseases Anti\u2010Plague Center, Federal Service on Consumers\u2019 Rights Protection and Human Well\u2010Being Surveillance Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yana","family":"Voytsekhovskaya","sequence":"additional","affiliation":[{"name":"Department of Molecular Diagnostics and Epidemiology Central Research Institute for Epidemiology, Federal Service on Consumers\u2019 Rights Protection and Human Well\u2010Being Surveillance Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Areg A.","family":"Totolian","sequence":"additional","affiliation":[{"name":"Department of Epidemiology Pasteur Institute, Federal Service on Consumers\u2019 Rights Protection and Human Well\u2010Being Surveillance Saint Petersburg Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,10,5]]},"reference":[{"key":"e_1_2_10_2_1","unstructured":"Coronavirus disease 2019.\u00a0https:\/\/www.who.int\/emergencies\/diseases\/novel-coronavirus-2019. Accessed May 2020."},{"key":"e_1_2_10_3_1","unstructured":"Coronavirus COVID\u201019\u00a0(2019\u2010nCoV).\u00a0https:\/\/mpsde4.mpslimited.com\/DigiEditpro\/DigiEditPage.aspx?FileName=14772471348010446663889.xml. Accessed May 20 2020."},{"key":"e_1_2_10_4_1","first-page":"145","article-title":"The epidemiological characteristics of an outbreak of 2019 novel coronavirus diseases (COVID\u201019) in China","volume":"41","author":"Epidemiology Working Group for NCIP Epidemic Response, Chinese Center for Disease Control and Prevention","year":"2020","journal-title":"Zhonghua Liu Xing Bing Xue Za Zhi"},{"issue":"4","key":"e_1_2_10_5_1","first-page":"119","article-title":"Epidemiology, virology, and clinical features of severe acute respiratory syndrome\u2010coronavirus\u20102 (SARS\u2010COV\u20102; coronavirus disease\u201019)","volume":"63","author":"Park SE","year":"2020","journal-title":"Korean J Pediatr"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/CM9.0000000000000722"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30154-9"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30251-8"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-020-05985-9"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-020-0695-z"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2012-7"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30183-5"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30211-7"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2020.1585"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.25693"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc2004973"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2002032"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(20)30134-1"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1148\/radiol.2020200702"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2020.25.3.2000045"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01739-19"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2008-3"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/d41587-020-00002-2"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00310-20"},{"key":"e_1_2_10_25_1","unstructured":"CDC 2019\u2014Novel Coronavirus (2019\u2010NCoV). Real\u2010Time RT\u2010PCR Diagnostic Panel For Emergency Use Only Instructions for Use;\u00a02019"},{"key":"e_1_2_10_26_1","unstructured":"Summary of NMPA approved novel coronavirus 2019\u2010nCoV test kits 2019\u2010nCoV test kits.http:\/\/english.nmpa.gov.cn\/2020-04\/03\/c_468570.htm. Accessed May 20 2020."},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.0c02624"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2018.12.002"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2015.11.007"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00713-06"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.12.3590-3594.1998"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2016.01.017"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0258(19980430)17:8<857::AID-SIM777>3.0.CO;2-E"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2020.104412"},{"key":"e_1_2_10_35_1","unstructured":"Chinese Center for Disease Control and Prevention.Primers and probes for detection 2019\u2010nCoV.https:\/\/www.who.int\/docs\/default-source\/coronaviruse\/whoinhouseassays.pdf?sfvrsn=de3a76aa_2. Accessed May 22 2020."},{"key":"e_1_2_10_36_1","volume-title":"Manual for the Laboratory\u2010Based Surveillance of Measles, Rubella, and Congenital Rubella Syndrome","author":"World Health Organization"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2606.200239"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2020.2565"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(20)30113-4"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2001191"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.26540","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jmv.26540","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.26540","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T03:52:01Z","timestamp":1693194721000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.26540"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,5]]},"references-count":39,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2021,3]]}},"alternative-id":["10.1002\/jmv.26540"],"URL":"https:\/\/doi.org\/10.1002\/jmv.26540","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10,5]]},"assertion":[{"value":"2020-08-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-09-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-10-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}