{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T06:19:51Z","timestamp":1790489991177,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,11,10]],"date-time":"2020-11-10T00:00:00Z","timestamp":1604966400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The uptake or expression of hepatitis B virus (HBV) proteins by dendritic cells (DCs) is considered important for disease outcome. Differential expression of microRNA (miRNA) may have a role in viral persistence and hepatocellular injury. The miRNA expression was investigated by microarray in DCs from different stages of HBV infection and liver disease namely, immune active (IA;\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200920); low replicative (LR;\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200920); HBeAg negative (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200920); acute viral hepatitis (AVH,\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200920) and healthy controls (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200920). miRNA levels were analyzed by unsupervised hierarchical clustering and principal component analyses and validated by quantitative polymerase Chain Reaction (qPCR). The miRNA\u2010messenger RNA (mRNA)regulatory networks identified 19 miRNAs and 12 target gene interactions in major histocompatibility complex\u00a0and other immune pathways. miR\u20102278, miR\u2010615\u20103p, and miR\u20103681\u20103p were downregulated in the IA group compared to healthy control, miR\u2010152\u20103p and miR\u20103613\u20103p in the LR group compared to IA group and miR\u2010152\u20103p and miR\u2010503\u20103p in HBe negative compared to LR group. However, miR\u20107\u20101\u20101\u20103p, miR\u2010192\u20105p, miR\u2010195\u20105p, and miR\u201032\u20105p in LR, miR\u2010342\u20103p, and miR\u2010940 in HBe negative, and miR\u201034a\u20105p, miR\u2010130b\u20103p, miR\u2010221\u20103p, miR\u2010320a, miR\u2010324\u20105p, and miR\u2010484 in AVH were upregulated. Further, qPCR confirmed changes in miRNA levels and their target genes associated with antigen processing and presentation. Thus, a deregulated network of miRNAs\u2010mRNAs in DCs seems responsible for an impaired immune response during HBV pathogenesis.\n                  <\/jats:p>","DOI":"10.1002\/jmv.26629","type":"journal-article","created":{"date-parts":[[2020,10,27]],"date-time":"2020-10-27T05:49:59Z","timestamp":1603777799000},"page":"3697-3706","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Identification of miRNAs associated with dendritic cell dysfunction during acute and chronic hepatitis B virus infection"],"prefix":"10.1002","volume":"93","author":[{"given":"Avishek Kumar","family":"Singh","sequence":"first","affiliation":[{"name":"Department of Molecular and Cellular Medicine Institute of Liver and Biliary Sciences  New Delhi India"},{"name":"Vascular and Interventional Translational Laboratory, Departments of Radiology Mayo Clinic Rochester Minnesota 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