{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T05:45:25Z","timestamp":1790315125610,"version":"4.1.0"},"reference-count":18,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,11,22]],"date-time":"2020-11-22T00:00:00Z","timestamp":1606003200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>As we strive towards the WHO goal of elimination of viral hepatitis as a public health threat by 2030, implementation of reliable, accurate diagnostic assays is crucial to identify those at risk of disease progression and those at risk of transmission. Ironically those at greatest risk of chronic hepatitis B are often in resource\u2010poor regions with limited access to testing, collection, storage, and\/or transportation of peripheral blood. The Xpert\u00ae HBV Viral Load assay provides an easy to use, convenient means of measuring load on GeneXpert platforms. In this study, the Xpert assay is evaluated against four commercially available high\u2010throughput assays for Hepatitis B virus (HBV) loads. In addition application of dried blood spots (DBS) for estimation of viral load is assessed on real\u2010world samples collected from a remote Pacific Island, Kiribati. A total of 107 serum\/plasma samples were tested in the Xpert HBV load assay and compared with the Abbott m2000, Alinity m, and Roche Cobas CAP\/CTM and 6800. Fifty\u2010three DBS were tested in the Xpert assay and compared with matching serum samples. Overall 82% serum\/plasma samples demonstrated good correlation between the Xpert and Roche and Abbott assays, to within 0.5 log<jats:sub>10<\/jats:sub> IU\/ml. The greatest discrepancies were seen at the limits of quantification of all assays. About 85.4% DBS gave estimable viral loads to within 1 log<jats:sub>10<\/jats:sub> IU\/ml of the serum load. The Xpert HBV viral load assay is recommended for all settings but particularly useful for resource\u2010poor settings. Utility of DBS with the Xpert assay provides a simple means for testing in remote settings.<\/jats:p>","DOI":"10.1002\/jmv.26662","type":"journal-article","created":{"date-parts":[[2020,11,11]],"date-time":"2020-11-11T12:04:49Z","timestamp":1605096289000},"page":"3707-3713","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Real\u2010world application of the Xpert\u00ae HBV viral load assay on serum and dried blood spots"],"prefix":"10.1002","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0507-678X","authenticated-orcid":false,"given":"Kathy","family":"Jackson","sequence":"first","affiliation":[{"name":"Victorian Infectious Diseases Reference Laboratory, Melbourne Health Doherty Institute for Infection and Immunity Melbourne Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosemary","family":"Tekoaua","sequence":"additional","affiliation":[{"name":"Ministry of Health and Medical Services Tungaru Central Hospital Tarawa Republic of Kiribati"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Li","sequence":"additional","affiliation":[{"name":"Victorian Infectious Diseases Reference Laboratory, Melbourne Health Doherty Institute for Infection and Immunity Melbourne Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"Locarnini","sequence":"additional","affiliation":[{"name":"Victorian Infectious Diseases Reference Laboratory, Melbourne Health Doherty Institute for Infection and Immunity Melbourne Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,11,22]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2468-1253(18)30056-6"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2016.06.083"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cld.2016.06.006"},{"key":"e_1_2_9_5_1","unstructured":"WHO. Global health sector strategy on viral hepatitis 2016\u20132021.https:\/\/www.whoint\/hepatitis\/strategy2016-2021\/ghss-hep\/en\/2016. Accessed November 19 2020."},{"key":"e_1_2_9_6_1","unstructured":"WHO. Prevention of mother\u2010to\u2010child transmission of hepatitis B virus: guidelines on antiviral prophylaxis in pregnancy. 2020.https:\/\/www.whoint\/publications\/i\/item\/978-92-4-000270-8. 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