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Effects of ESV infection on replication of DENV were explored in vitro and in vivo, analyzing protein, RNA genome expression, and plaque formation. ESV and DENV simultaneous coinfection did not block protein synthesis from either virus but did result in inhibition of DENV replication in mosquito cells. Furthermore, ESV superinfected with DENV resulted in inhibition of DENV replication and spread in <jats:italic>A. aegypti<\/jats:italic>, thus reducing vector competence. Tissue culture experiments on viral kinetics of ESV and DENV coinfection showed that neither virus significantly affects the replication of the other in Vero, HeLa, or HEK cells. Hence, ESV blocks DENV replication in insect cells, but not the mammalian cells evaluated here. Our study provides new insights into ESV\u2010induced suppression of DENV, a globally important pathogen impacting public health.<\/jats:p>","DOI":"10.1002\/jmv.26686","type":"journal-article","created":{"date-parts":[[2020,11,21]],"date-time":"2020-11-21T09:29:25Z","timestamp":1605950965000},"page":"3362-3373","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Mosquito\u2010infecting virus Espirito Santo virus inhibits replication and spread of dengue virus"],"prefix":"10.1002","volume":"93","author":[{"given":"Avian V.","family":"White","sequence":"first","affiliation":[{"name":"Department of Health Education and Promotion Environmental Health Sciences Program, College of Health and Human Performance East Carolina University Greenville North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Brody School of Medicine East Carolina University Greenville North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordan M.","family":"Mazzara","sequence":"additional","affiliation":[{"name":"Department of Health Education and Promotion Environmental Health Sciences Program, College of Health and Human Performance East Carolina University Greenville North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel L.","family":"Roper","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Brody School of Medicine East Carolina University Greenville North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0072-9251","authenticated-orcid":false,"given":"Stephanie L.","family":"Richards","sequence":"additional","affiliation":[{"name":"Department of Health Education and Promotion Environmental Health Sciences Program, College of Health and Human Performance East Carolina University Greenville North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,12]]},"reference":[{"key":"e_1_2_9_2_1","unstructured":"Centers for Disease Control. 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