{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T20:21:05Z","timestamp":1771273265679,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T00:00:00Z","timestamp":1606780800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100010580","name":"Department of Science and Technology, Hubei Provincial People's Government","doi-asserted-by":"publisher","award":["2019ZYYD066"],"award-info":[{"award-number":["2019ZYYD066"]}],"id":[{"id":"10.13039\/501100010580","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cervical cancer is the fourth most common malignant tumor in women worldwide and is closely related to human papillomavirus (HPV). Women have the highest susceptibility to HPV\u201052 type in Jingzhou, China. In this study, E6\u2010E7 sequences of 183 HPV\u201052 positive samples were amplified by a polymerase chain reaction and sequenced. HPV\u201052 E6\u2010E7 gene variations were analyzed. The phylogenetic tree was constructed using the Kimura 2\u2010parameter method. The secondary structure of the protein was analyzed. The selective pressure to E6\u2010E7 genes was estimated using PAML. In addition, the B cell epitopes of the E6\u2010E7 sequences in HPV\u201052 were predicted by the ABCpred server. In E6 sequences, 15 single nucleotide variants were observed, including 6 nonsynonymous variants and 9 synonymous variants. In E7 sequences, 19 single nucleotide variants occurred, including 10 nonsynonymous variants and 9 synonymous variants. Six\u00a0amino acid\u00a0variants, including 3 nonconservative substitutions, were found in sequences encoding the alpha helix. Eight amino acid variants, including three nonconservative substitutions, occurred in sequences encoding the strand. Through phylogenetic analysis, the E6\u2010E7 sequences were mainly distributed in B lineage. In HPV\u201052 E6\u2010E7 sequences, no positively selected site was found. The nonconservative substitutions, such as K93R, K93E in E6, T37I, and D38N in E7, affected multiple hypothetical epitopes in the B cell. This study provides information for the investigation of HPV epidemic characters. The discovery of new variants of HPV\u201052 may lay the basis for the development of the virus diagnosis, further study of cervical cancer, and vaccine design in Central China.<\/jats:p>","DOI":"10.1002\/jmv.26690","type":"journal-article","created":{"date-parts":[[2020,11,24]],"date-time":"2020-11-24T12:54:52Z","timestamp":1606222492000},"page":"3849-3856","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Genetic variation of E6 and E7 genes of human papillomavirus 52 from Central China"],"prefix":"10.1002","volume":"93","author":[{"given":"Shuo","family":"Li","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine Jingzhou Central Hospital, The Second Clinical Medical College, Yangtze University Jingzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mengxia","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Jingzhou 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