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Molecular SARS\u2010CoV\u20102 diagnostics based on nucleic acids (NA) derived from oro\u2010 or nasopharyngeal swabs constitute the current gold standard. Given the importance of test results, it is crucial to assess the quality of the underlying swab samples and NA extraction procedures. We determined NA concentrations in clinical samples used for SARS\u2010CoV\u20102 testing applying an NA\u2010specific dye. In comparison to cut\u2010offs defined by SARS\u2010CoV\u20102\u2010positive samples, internal positive controls, and references from a federal laboratory, 90.85% (923 of 1016) of swabs contained NA concentrations enabling SARS\u2010CoV\u20102 recognition. Swabs collected by local health authorities and the central emergency department either had significantly higher NA concentrations or were less likely to exhibit insufficient quality, arguing in favor of sampling centers with routined personnel. Interestingly, samples taken from females had significantly higher NA concentrations than those from males. Among eight longitudinal patient sample sets with intermitting negative quantitative reverse transcription polymerase chain reaction results, two showed reduced NA concentrations in negative specimens. The herein described fluorescence\u2010based NA quantification approach is immediately applicable to evaluate swab qualities, optimize sampling strategies, identify patient\u2010specific differences, and explain some peculiar test results including intermittent negative samples with low NA concentrations.<\/jats:p>","DOI":"10.1002\/jmv.26706","type":"journal-article","created":{"date-parts":[[2020,12,2]],"date-time":"2020-12-02T14:00:00Z","timestamp":1606917600000},"page":"2848-2856","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Over 90% of clinical swabs used for SARS\u2010CoV\u20102 diagnostics contain sufficient nucleic acid concentrations"],"prefix":"10.1002","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1976-576X","authenticated-orcid":false,"given":"Robin L.","family":"Klingen","sequence":"first","affiliation":[{"name":"Institute for Virology, University Hospital Essen University of Duisburg\u2010Essen Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Katschinski","sequence":"additional","affiliation":[{"name":"Institute for Virology, University Hospital Essen University of Duisburg\u2010Essen Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olympia E.","family":"Anastasiou","sequence":"additional","affiliation":[{"name":"Institute for Virology, University Hospital Essen University of Duisburg\u2010Essen Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. Stefan","family":"Ross","sequence":"additional","affiliation":[{"name":"Institute for Virology, University Hospital Essen University of Duisburg\u2010Essen Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulf","family":"Dittmer","sequence":"additional","affiliation":[{"name":"Institute for Virology, University Hospital Essen University of Duisburg\u2010Essen Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2733-3732","authenticated-orcid":false,"given":"Vu Thuy Khanh","family":"Le\u2010Trilling","sequence":"additional","affiliation":[{"name":"Institute for Virology, University Hospital Essen University of Duisburg\u2010Essen Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3659-3541","authenticated-orcid":false,"given":"Mirko","family":"Trilling","sequence":"additional","affiliation":[{"name":"Institute for Virology, University Hospital Essen University of Duisburg\u2010Essen Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,2,16]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(20)30120-1"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmii.2020.03.013"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2020.25.26.2001214"},{"key":"e_1_2_10_5_1","doi-asserted-by":"crossref","unstructured":"New York City Department of H Mental Hygiene C\u2010RT. 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