{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T17:11:19Z","timestamp":1777569079760,"version":"3.51.4"},"reference-count":16,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,12,23]],"date-time":"2020-12-23T00:00:00Z","timestamp":1608681600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      There are very few studies in search of an alternate and convenient diagnostic tool which can substitute nasopharyngeal swab (NPS) specimen for detection of SARS\u2010CoV\u20102. In the study we analyzed, the comparison and agreement between the feasibility of using the saliva in comparison to NPS for diagnosis of SARS\u2010CoV\u20102. A total number of 74 patients were enrolled for this study. We analyzed and compared the NPS and saliva specimen collected within 48\u2009h after the symptom onset. We carried out real\u2010time quantitative polymerase chain reaction, gene sequencing for the detection and determination SARS\u2010CoV\u20102 specific genes. Phylogenetic tree was constructed to establish the isolation of viral RNA from saliva. We used the Bland\u2013Altman model to identify the agreement between two specimens. This study showed a lower cycle threshold (\n                      <jats:italic>C<\/jats:italic>\n                      <jats:sub>T<\/jats:sub>\n                      ) mean value for the detection of SARS\u2010CoV\u20102\n                      <jats:italic>ORF1<\/jats:italic>\n                      gene (mean, 27.07; 95% confidence interval [CI], 25.62 to 28.52) in saliva methods than that of NPS (mean 28.24; 95% CI, 26.62 to 29.85) specimen although the difference is statistically nonsignificant (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&gt;\u2009.05). Bland\u2013Altman analysis produced relatively smaller bias and high agreement between these two clinical specimens. Phylogenetic analysis with the\n                      <jats:italic>RdRp<\/jats:italic>\n                      and\n                      <jats:italic>S<\/jats:italic>\n                      gene confirmed the presence of SARS\u2010CoV\u20102 in the saliva samples. Saliva represented a promising tool in COVID\u201019 diagnosis and the collection method would reduce the exposure risk of frontline health workers which is one of the major concerns in primary healthcare settings.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jmv.26719","type":"journal-article","created":{"date-parts":[[2020,12,10]],"date-time":"2020-12-10T09:44:01Z","timestamp":1607593441000},"page":"2529-2533","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Saliva for diagnosis of SARS\u2010CoV\u20102: First report from India"],"prefix":"10.1002","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5199-5288","authenticated-orcid":false,"given":"Debdutta","family":"Bhattacharya","sequence":"first","affiliation":[{"name":"Department of Microbiology ICMR\u2010Regional Medical Research Centre (Dept. of Health Research, Ministry of Health &amp; Family Welfare, Govt. of India) Bhubaneswar India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Debaprasad","family":"Parai","sequence":"additional","affiliation":[{"name":"Department of Microbiology ICMR\u2010Regional Medical Research Centre (Dept. of Health Research, Ministry of Health &amp; Family Welfare, Govt. of India) Bhubaneswar India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Usha K.","family":"Rout","sequence":"additional","affiliation":[{"name":"Department of Microbiology ICMR\u2010Regional Medical Research Centre (Dept. of Health Research, Ministry of Health &amp; Family Welfare, Govt. of India) Bhubaneswar India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pujarini","family":"Dash","sequence":"additional","affiliation":[{"name":"Department of Microbiology ICMR\u2010Regional Medical Research Centre (Dept. of Health Research, Ministry of Health &amp; Family Welfare, Govt. of India) Bhubaneswar India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rashmi R.","family":"Nanda","sequence":"additional","affiliation":[{"name":"Department of Microbiology ICMR\u2010Regional Medical Research Centre (Dept. of Health Research, Ministry of Health &amp; Family Welfare, Govt. of India) Bhubaneswar India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Girish C.","family":"Dash","sequence":"additional","affiliation":[{"name":"Department of Microbiology ICMR\u2010Regional Medical Research Centre (Dept. of Health Research, Ministry of Health &amp; Family Welfare, Govt. of India) Bhubaneswar India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Srikanta","family":"Kanungo","sequence":"additional","affiliation":[{"name":"Department of Microbiology ICMR\u2010Regional Medical Research Centre (Dept. of Health Research, Ministry of Health &amp; Family Welfare, Govt. of India) Bhubaneswar India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Subrata K.","family":"Palo","sequence":"additional","affiliation":[{"name":"Department of Microbiology ICMR\u2010Regional Medical Research Centre (Dept. of Health Research, Ministry of Health &amp; Family Welfare, Govt. of India) Bhubaneswar India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sidhartha","family":"Giri","sequence":"additional","affiliation":[{"name":"Department of Microbiology ICMR\u2010Regional Medical Research Centre (Dept. of Health Research, Ministry of Health &amp; Family Welfare, Govt. of India) Bhubaneswar India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hari R.","family":"Choudhary","sequence":"additional","affiliation":[{"name":"Department of Microbiology ICMR\u2010Regional Medical Research Centre (Dept. of Health Research, Ministry of Health &amp; Family Welfare, Govt. of India) Bhubaneswar India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaya S.","family":"Kshatri","sequence":"additional","affiliation":[{"name":"Department of Microbiology ICMR\u2010Regional Medical Research Centre (Dept. of Health Research, Ministry of Health &amp; Family Welfare, Govt. of India) Bhubaneswar India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jyotirmayee","family":"Turuk","sequence":"additional","affiliation":[{"name":"Department of Microbiology ICMR\u2010Regional Medical Research Centre (Dept. of Health Research, Ministry of Health &amp; Family Welfare, Govt. of India) Bhubaneswar India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bijay K.","family":"Mishra","sequence":"additional","affiliation":[{"name":"Department of Microbiology ICMR\u2010Regional Medical Research Centre (Dept. of Health Research, Ministry of Health &amp; Family Welfare, Govt. of India) Bhubaneswar India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rajesh K.","family":"Lenka","sequence":"additional","affiliation":[{"name":"Department of Microbiology IMS &amp; SUM Hospital Bhubaneswar India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saroj","family":"Dash","sequence":"additional","affiliation":[{"name":"Department of Microbiology ICMR\u2010Regional Medical Research Centre (Dept. of Health Research, Ministry of Health &amp; Family Welfare, Govt. of India) Bhubaneswar India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanghamitra","family":"Pati","sequence":"additional","affiliation":[{"name":"Department of Microbiology ICMR\u2010Regional Medical Research Centre (Dept. of Health Research, Ministry of Health &amp; Family Welfare, Govt. of India) Bhubaneswar India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,12,23]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/d41586-020-01865-w"},{"key":"e_1_2_8_3_1","unstructured":"World Health Organization. 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Interim Guidelines for Collecting Handling and Testing Clinical Specimens for COVID\u201019;2020.https:\/\/www.cdc.gov\/coronavirus\/2019-ncov\/lab\/guidelines-clinical-specimens.html"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMvcm2010260"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoto.2020.3579"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2020.104821"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2018.06.009"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41368-020-0080-z"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30154-9"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msr121"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.25721"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm9051491"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2468-2667(20)30164-X"},{"key":"e_1_2_8_15_1","first-page":"153","article-title":"Safety management of nasopharyngeal specimen collection from suspected cases of coronavirus disease 2019","volume":"7","author":"Qian Y","year":"2020","journal-title":"Int J Nurs Sci"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa149"},{"issue":"20","key":"e_1_2_8_17_1","first-page":"30278","article-title":"Saliva sample as a non\u2010invasive specimen for the diagnosis of coronavirus disease 2019: a cross\u2010sectional study","volume":"1198","author":"Pasomsub E","year":"2020","journal-title":"Clin Microbiol Infect"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.26719","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jmv.26719","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.26719","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,16]],"date-time":"2024-07-16T06:00:25Z","timestamp":1721109625000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.26719"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12,23]]},"references-count":16,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2021,4]]}},"alternative-id":["10.1002\/jmv.26719"],"URL":"https:\/\/doi.org\/10.1002\/jmv.26719","archive":["Portico"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.09.11.20192591","asserted-by":"object"}]},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,12,23]]},"assertion":[{"value":"2020-10-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}