{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T21:45:19Z","timestamp":1759009519975,"version":"3.40.5"},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,1,5]],"date-time":"2021-01-05T00:00:00Z","timestamp":1609804800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS\u2010COV\u20102) cases is based on the count of real\u2010time reverse transcription\u2010plymerase chain reaction (RT\u2010PCR) positive people. Viral load by real\u2010time RT\u2010PCR has been suggested as a biomarker of the SARS\u2010CoV\u20102 infection. However, the association of viral load and severity of the disease is not yet resolved. Nasopharyngeal samples from 458 patients were tested by RT\u2010PCR for SARS\u2010CoV\u20102 diagnosis. Relative quantitation was made by the comparative threshold cycle (\u0394\u0394<jats:italic>C<\/jats:italic><jats:sub>t<\/jats:sub>) formula between ORF1ab viral and RNase P housekeeping genes. Absolute viral load was calculate using a reference positive control. Most prevalent clinical signs were cough (75.8%), myalgia (66.7%), and fever (48.5%). Hypertension (18.2%), neurological diseases (15.1%), and asthma and hypothyroidism (12.1%) were most frequent comorbidities. Fever, either as an exclusive symptom or combined with others, was associated with high viral loads ( range, 35.65\u2013155.16; 4.25\u20134.89 log<jats:sub>10<\/jats:sub> RNA copies\/test]). During the first week after onset of symptoms in mild patients up to 60 years\u2010old was detected the peak of viral load. Children under 10 years old have a high viral load (313.84; 2.50) in the first 2 days postinfection with a sharp decline thereafter. Cases between 10 and 49 years old mostly showed low and moderate viral load during the first 2 days postinfection (range, 0.03 to 17.24; \u22121.50 to 1.24). Patients over 60 years old have high viral load up to the second week after the onset of symptoms (range, 25.32\u2013155.42; 1.40\u20132.19), indicating the longer presence of the virus in them. These findings suggest the viral load in nasopharyngeal swabs would help to monitor the SARS\u2010CoV\u20102 infection in mild coronavirus disease 2019 cases.<\/jats:p>","DOI":"10.1002\/jmv.26761","type":"journal-article","created":{"date-parts":[[2020,12,26]],"date-time":"2020-12-26T09:25:07Z","timestamp":1608974707000},"page":"2439-2445","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Quantitative detection of SARS\u2010CoV\u20102 RNA in nasopharyngeal samples from infected patients with mild disease"],"prefix":"10.1002","volume":"93","author":[{"given":"Patricia","family":"Bustos","sequence":"first","affiliation":[{"name":"Section of Respiratory Virus, Subdepartment of Viral Diseases, Biomedical Department Public Health Institute of Chile  Santiago Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolina","family":"Tambley","sequence":"additional","affiliation":[{"name":"Section of Respiratory Virus, Subdepartment of Viral Diseases, Biomedical Department Public Health Institute of Chile  Santiago Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandra","family":"Acevedo","sequence":"additional","affiliation":[{"name":"Section of Respiratory Virus, Subdepartment of Viral Diseases, Biomedical Department Public Health Institute of Chile  Santiago Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Winston","family":"Andrade","sequence":"additional","affiliation":[{"name":"Section of Respiratory Virus, Subdepartment of Viral Diseases, Biomedical Department Public Health Institute of Chile  Santiago Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriel","family":"Leal","sequence":"additional","affiliation":[{"name":"Section of Respiratory Virus, Subdepartment of Viral Diseases, Biomedical Department Public Health Institute of Chile  Santiago Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deyanira","family":"Vidal","sequence":"additional","affiliation":[{"name":"Section of Oncogenic Virus, Subdepartment of Viral Diseases, Biomedical Department Public Health Institute of Chile  Santiago Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco","family":"Rold\u00e1n","sequence":"additional","affiliation":[{"name":"Section of Oncogenic Virus, Subdepartment of Viral Diseases, Biomedical Department Public Health Institute of Chile  Santiago Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodrigo","family":"Fasce","sequence":"additional","affiliation":[{"name":"Section of Respiratory Virus, Subdepartment of Viral Diseases, Biomedical Department Public Health Institute of Chile  Santiago Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5299-7928","authenticated-orcid":false,"given":"Eugenio","family":"Ram\u00edrez","sequence":"additional","affiliation":[{"name":"Section of Oncogenic Virus, Subdepartment of Viral Diseases, Biomedical Department Public Health Institute of Chile  Santiago Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,1,5]]},"reference":[{"key":"e_1_2_9_2_1","unstructured":"COVID\u201019 Dashboard by the Center for Systems Science and Engineering (CSSE) at Johns Hopkins Universty (JHU).https:\/\/gisanddata.maps.arcgis.com\/apps\/opsdashboard\/index.html#\/bda7594740fd40299423467b48e9ecf6. 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