{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T18:21:50Z","timestamp":1773339710052,"version":"3.50.1"},"reference-count":11,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,1,11]],"date-time":"2021-01-11T00:00:00Z","timestamp":1610323200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>SARS\u2010CoV\u20102 nucleocapsid (N) protein has been proposed as a good vaccine target. N\u2010specific T cells were observed in SARS\u2010CoV\u20102 N immunized mice and COVID\u201019 convalescents. It is of importance to identify the T cell responses triggered by SARS\u2010CoV\u20102 N protein. Intradermal immunization with SARS\u2010CoV N protein was demonstrated to elicit non\u2010protective T cell responses which may be avoided by intranasal vaccination. Therefore, we conducted intranasal vaccination of BALB\/c mice with recombinant adenovirus type\u20105 expressing SARS\u2010CoV\u20102 N protein. Such procedure induced CD8 T cell responses in the lung. Meanwhile CD4 T cell responses were observed in the spleen, which was associated with robust antibody production. Our study further supports the notion that SARS\u2010CoV\u20102 N protein can work as a target for vaccine development.<\/jats:p>","DOI":"10.1002\/jmv.26769","type":"journal-article","created":{"date-parts":[[2021,1,2]],"date-time":"2021-01-02T12:21:22Z","timestamp":1609590082000},"page":"1923-1925","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["SARS\u2010CoV\u20102 nucleocapsid protein intranasal inoculation induces local and systemic T cell responses in mice"],"prefix":"10.1002","volume":"93","author":[{"given":"Jia","family":"He","sequence":"first","affiliation":[{"name":"Beijing Institute of Basic Medical Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing\u2010Ru","family":"Huang","sequence":"additional","affiliation":[{"name":"Beijing Institute of Basic Medical Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yao\u2010Lin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Beijing Institute of Basic Medical Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8649-8035","authenticated-orcid":false,"given":"Jiyan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Beijing Institute of Basic Medical Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,1,11]]},"reference":[{"key":"e_1_2_1_8_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00647-20"},{"key":"e_1_2_1_8_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01279-20"},{"key":"e_1_2_1_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2020.10.006"},{"key":"e_1_2_1_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2020.04.023"},{"key":"e_1_2_1_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2016.02.063"},{"key":"e_1_2_1_8_7_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.181.9.6337"},{"key":"e_1_2_1_8_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01049-10"},{"key":"e_1_2_1_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2012.11"},{"key":"e_1_2_1_8_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00823-14"},{"key":"e_1_2_1_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2016.05.006"},{"key":"e_1_2_1_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2020.04.009"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.26769","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jmv.26769","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.26769","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,16]],"date-time":"2024-07-16T10:04:56Z","timestamp":1721124296000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.26769"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1,11]]},"references-count":11,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2021,4]]}},"alternative-id":["10.1002\/jmv.26769"],"URL":"https:\/\/doi.org\/10.1002\/jmv.26769","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1,11]]},"assertion":[{"value":"2020-11-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-01-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}