{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T11:17:57Z","timestamp":1779103077335,"version":"3.51.4"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,1,22]],"date-time":"2021-01-22T00:00:00Z","timestamp":1611273600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>An optimal clinical specimen for accurate detection of severe acute respiratory syndrome coronavirus 2 (SARS\u2010CoV\u20102) by minimizing the usage of consumables and reduce hazard exposure to healthcare workers is an urgent\u00a0priority. The diagnostic performance of SARS\u2010CoV\u20102 detection between healthcare worker\u2010collected nasopharyngeal and oropharyngeal (NP\u2009+\u2009OP) swabs and patient performed self\u2010collected random saliva was assessed. Paired NP\u2009+\u2009OP swabs and random saliva were collected and processed within 48\u2009h of specimen collection from two cohort studies\u00a0which recruited 562 asymptomatic adult candidates. Real\u2010time reverse\u2010transcription polymerase chain reaction\u00a0targeting Open reading frame 1a (ORF1a) and nucleocapsid (N) genes was performed and the results were compared. Overall, 65 of 562 (28.1%) candidates tested positive for COVID\u201019 based on random saliva, NP\u2009+\u2009OP swabs, or both testing techniques. The detection rate of SARS\u2010CoV\u20102 was higher in random saliva compared to NP\u2009+\u2009OP testing (92.3%; 60\/65 vs. 73.8%; 48\/65; <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). The estimated sensitivity and specificity of random saliva were higher than NP\u2009+\u2009OP swabs (95.0; 99.9 vs. 72.2; 99.4). The <jats:italic>C<\/jats:italic><jats:sub>t\u00a0<\/jats:sub>values of ORF1a and N genes were significantly lower in random saliva compared to NP\u2009+\u2009OP swabs specimens.\u00a0Our findings demonstrate that random saliva is an alternative diagnostic specimen for the detection of SARS\u2010CoV\u20102. Self\u2010collected random oropharyngeal saliva is a valuable specimen that provides accurate SARS\u2010CoV\u20102 surveillance testing of a community.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.26773","type":"journal-article","created":{"date-parts":[[2021,1,4]],"date-time":"2021-01-04T13:54:07Z","timestamp":1609768447000},"page":"2461-2466","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["COVID\u201019 screening test by using random oropharyngeal saliva"],"prefix":"10.1002","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9331-0486","authenticated-orcid":false,"given":"Mohan","family":"Rao","sequence":"first","affiliation":[{"name":"Infectious Disease Research Centre, Institute for Medical Research Ministry of Health Malaysia  Jalan Pahang Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fairuz A.","family":"Rashid","sequence":"additional","affiliation":[{"name":"Infectious Disease Research Centre, Institute for Medical Research Ministry of Health Malaysia  Jalan Pahang Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fashihah S. 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L.","family":"Aren","sequence":"additional","affiliation":[{"name":"Kota Setar District Health Office  Kedah Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shareh A. 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