{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T19:55:08Z","timestamp":1774641308545,"version":"3.50.1"},"reference-count":24,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,2,23]],"date-time":"2021-02-23T00:00:00Z","timestamp":1614038400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007128","name":"Natural Science Foundation of Shaanxi Province","doi-asserted-by":"publisher","award":["2020JM\u2010655"],"award-info":[{"award-number":["2020JM\u2010655"]}],"id":[{"id":"10.13039\/501100007128","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007128","name":"Natural Science Foundation of Shaanxi Province","doi-asserted-by":"publisher","award":["2020JM\u2010662"],"award-info":[{"award-number":["2020JM\u2010662"]}],"id":[{"id":"10.13039\/501100007128","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Influenza virus cause seasonal influenza epidemic and seriously sporadic influenza pandemic outbreaks. Hemagglutinin (HA) is an important target in the therapeutic treatment and diagnostic detection of the influenza virus. Variation in the sialic acid receptor binding site leads to strain\u2010specific binding and results in different binding modes to the host receptors. Here, we evaluated the neutralizing activity and hemagglutination inhibition activity of a prepared murine anti\u2010H1N1 monoclonal antibody PR8\u201023. Then we identified the epitope peptide of antibody PR8\u201023 by phage display technique from phage display peptide libraries. The identified epitope, 63\u2010IAPLQLGKCNIA\u201074, containing two \u03b1\u2010helix and two \u03b2\u2010fold located at the footprint of the sialoglycan receptor on the RBS in the globular head domain of HA. It broads the growing arsenal of motifs for the amino acids on the globular head domain of HA in sialic acid receptor binding site and neutralizing antibody production.<\/jats:p>","DOI":"10.1002\/jmv.26779","type":"journal-article","created":{"date-parts":[[2021,1,10]],"date-time":"2021-01-10T16:54:00Z","timestamp":1610297640000},"page":"3508-3515","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Neutralizing antibody PR8\u201023 targets the footprint of the sialoglycan receptor binding site of H1N1 hemagglutinin"],"prefix":"10.1002","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1503-6087","authenticated-orcid":false,"given":"Liting","family":"Yan","sequence":"first","affiliation":[{"name":"Central Laboratory \u00a0Shaanxi Provincial People's Hospital Xi'an China"},{"name":"Research Center of Cell Immunological Engineering and Technology of Shaanxi Province Xi'an 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of Lanzhou University Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingying","family":"Sun","sequence":"additional","affiliation":[{"name":"Central Laboratory \u00a0Shaanxi Provincial People's Hospital Xi'an China"},{"name":"Research Center of Cell Immunological Engineering and Technology of Shaanxi Province Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyan","family":"Huang","sequence":"additional","affiliation":[{"name":"Central Laboratory \u00a0Shaanxi Provincial People's Hospital Xi'an China"},{"name":"Research Center of Cell Immunological Engineering and Technology of Shaanxi Province Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Penghua","family":"Zhao","sequence":"additional","affiliation":[{"name":"Central Laboratory \u00a0Shaanxi Provincial People's Hospital Xi'an China"},{"name":"Research Center of Cell Immunological Engineering and Technology of Shaanxi Province Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Xie","sequence":"additional","affiliation":[{"name":"College of Life Sciences Key Laboratory of Resource Biology and Biotechnology in Western China Ministry of Education, Northwest University Xi'an China"},{"name":"Department of Translational Medicine Institute of Integrated Medical Information Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Hu","sequence":"additional","affiliation":[{"name":"Central Laboratory \u00a0Shaanxi Provincial People's Hospital Xi'an China"},{"name":"Research Center of Cell Immunological Engineering and Technology of Shaanxi Province Xi'an 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