{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T14:10:26Z","timestamp":1782483026876,"version":"3.54.5"},"reference-count":18,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,2,9]],"date-time":"2021-02-09T00:00:00Z","timestamp":1612828800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The ongoing coronavirus disease 2019 (Covid\u201019) pandemic has been rapidly spreading throughout the world with confirmed case numbers already exceeding 75 million. Although nasopharyngeal swabs are the most commonly utilized samples for based severe acute respiratory syndrome coronavirus 2 (SARS\u2010CoV\u20102) RNA detection, collecting these specimens requires healthcare workers and necessitates the use of personal protective equipment as\u00a0it presents a nosocomial transmission risk.\u00a0We aimed to assess the diagnostic value of saliva samples in mildly symptomatic and asymptomatic patients with confirmed Covid\u201019.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We performed\u00a0a cohort study to validate the use of saliva for SARS\u2010CoV\u20102 detection in mildly symptomatic and asymptomatic patients with a confirmed diagnosis of Covid\u201019. Saliva samples of the patients were analyzed by reverse\u2010transcriptase polymerase chain reaction (RT\u2010PCR).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In May 2020, 28 asymptomatic and 25 mildly symptomatic patients were enrolled in the study. The median age was 37 years (range 4\u201370). None of the patients had a fever on presentation.\u00a0Among 53 patients with SARS\u2010CoV\u20102 detected in the nasopharyngeal sample, the real\u2010time RT\u2010PCR was positive in the saliva specimens in 48 (90.56%) patients. The mean cycle threshold (CT) values for nasopharyngeal and saliva specimens (27.80\u2009\u00b1\u20093.44 and 30.64\u2009\u00b1\u20092.83, respectively) were significantly correlated between the two sample types (<jats:italic>p<\/jats:italic>\u2009=\u2009.016).\u00a0The mean CT values of nasopharyngeal and saliva samples in mildly symptomatic and asymptomatic patients (27.18\u2009\u00b1\u20093.53 and 30.24\u2009\u00b1\u20093.29 vs. 28.36\u2009\u00b1\u20093.31 and 30.98\u2009\u00b1\u20092.39, respectively) were not significantly different (<jats:italic>p<\/jats:italic>\u2009=\u2009.236 and <jats:italic>p<\/jats:italic>\u2009=\u2009.733, respectively).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Saliva specimens can be considered as a reliable and less resource\u2010intensive alternative to nasopharyngeal specimens for screening asymptomatic SARS\u2010CoV\u20102 infections.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.26821","type":"journal-article","created":{"date-parts":[[2021,1,27]],"date-time":"2021-01-27T02:20:31Z","timestamp":1611714031000},"page":"2932-2937","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Saliva samples for detection of SARS\u2010CoV\u20102 in mildly symptomatic and asymptomatic patients"],"prefix":"10.1002","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7583-8081","authenticated-orcid":false,"given":"Emin Ediz","family":"Tutuncu","sequence":"first","affiliation":[{"name":"Department of Infectious Disease and Clinical Microbiology,\u00a0Faculty of Medicine Kafkas University  Kars Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8320-9453","authenticated-orcid":false,"given":"Didem","family":"Ozgur","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine Kafkas University  Kars Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7803-1462","authenticated-orcid":false,"given":"Murat","family":"Karamese","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,2,9]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.46234\/ccdcw2020.017"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2001017"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30154-9"},{"key":"e_1_2_8_5_1","unstructured":"World Health Organization.Coronavirus disease (COVID\u201019) situation report.https:\/\/www.who.int\/docs\/default-source\/coronaviruse\/20200630-covid-19-sitrep-162.pdf?sfvrsn=e00a5466_2(accessed December 20 2020)."},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc2001737"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2020.25.3.2000045"},{"key":"e_1_2_8_8_1","unstructured":"Centers for Disease Control and Pevention. Interim Guidelines for Collecting Handling and Testing Clinical Specimens from Persons for Coronavirus Disease 2019 (COVID\u201019).www.cdc.gov\/coronavirus\/2019-nCoV\/lab\/guidelines-clinical-specimens.html(accessed June 29 2020)."},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01704-16"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/emi.2017.35"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.7326\/M20-1301"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2020.04.005"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(20)30196-1"},{"key":"e_1_2_8_14_1","article-title":"Saliva is more sensitive for SARS\u2010CoV\u20102 detection in COVID\u201019 patients than nasopharyngeal swabs","author":"Wyllie AL","year":"2020","journal-title":"medRxiv"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMp2006141"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa149"},{"key":"e_1_2_8_17_1","article-title":"Saliva as a candidate for COVID\u201019 diagnostic testing: a meta\u2010analysis","author":"Czumbel LM","year":"2020","journal-title":"medRxiv"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(20)30232-2"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abb3221"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.26821","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jmv.26821","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.26821","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T12:07:09Z","timestamp":1693310829000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.26821"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,2,9]]},"references-count":18,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2021,5]]}},"alternative-id":["10.1002\/jmv.26821"],"URL":"https:\/\/doi.org\/10.1002\/jmv.26821","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,2,9]]},"assertion":[{"value":"2020-12-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-01-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-02-09","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}