{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T19:05:00Z","timestamp":1781723100733,"version":"3.54.5"},"reference-count":12,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2021,2,15]],"date-time":"2021-02-15T00:00:00Z","timestamp":1613347200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Conjunctivitis is a frequent ocular disorder caused by human adenoviruses (HAdVs). Only a few of the 45 HAdV\u2010D species are associated with epidemic keratoconjunctivitis, including HAdV\u2010D8. Nosocomial outbreaks due to HAdV\u2010D8 have been rarely described, because keratoconjunctivitis cases are clinically diagnosed and treated without having to characterize the causative agent. Moreover, molecular typing is tedious when using classical techniques.\u00a0In this study, a hospital outbreak of conjunctivitis caused by HAdV\u2010D8 was characterized using the recently developed whole\u2010genome sequencing (WGS) method. Of the 363 patients attending the Ophthalmology Department between July 13 and August 13, 2018, 36 may have acquired intrahospital conjunctivitis. Also, 11 of 22 samples sent to the Virology section were selected for WGS analysis.\u00a0The WGS results revealed that 10 out of 11 HAdV\u2010D8 strains were closely related. The remaining strain (Case 28) was more similar to a strain from an outbreak in Germany obtained from a public sequence database. WGS results showed that outbreak HAdV\u2010D8 strains had a minimum percentage of identity of 94.3%.\u00a0WGS is useful in a clinical setting, because it avoids carrying out viral culture or specific polymerase chain reaction\u00a0sequencing. The public availability of sequence reads makes it easier to compare clusters in circulation. In conclusion, WGS can play an important role in standard routines to describe viral outbreaks.<\/jats:p>","DOI":"10.1002\/jmv.26850","type":"journal-article","created":{"date-parts":[[2021,2,6]],"date-time":"2021-02-06T09:22:59Z","timestamp":1612603379000},"page":"4840-4845","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Whole\u2010genome analysis to describe a human adenovirus D8 conjunctivitis outbreak in a tertiary hospital"],"prefix":"10.1002","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2781-6926","authenticated-orcid":false,"given":"Elisenda","family":"Miro","sequence":"first","affiliation":[{"name":"Department of Microbiology, Hospital de la Santa Creu i Sant Pau Institut d'Investigaci\u00f3 Biom\u00e8dica Sant Pau  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margarita","family":"Del Cuerpo","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Hospital de la Santa Creu i Sant Pau Institut d'Investigaci\u00f3 Biom\u00e8dica Sant Pau  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Rubio","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Hospital de la Santa Creu i Sant Pau Institut d'Investigaci\u00f3 Biom\u00e8dica Sant Pau  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla","family":"Berengua","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Hospital de la Santa Creu i Sant Pau Institut d'Investigaci\u00f3 Biom\u00e8dica Sant Pau  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Montserrat","family":"Espa\u00f1ol","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Hospital de la Santa Creu i Sant Pau Institut d'Investigaci\u00f3 Biom\u00e8dica Sant Pau  Barcelona 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