{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T14:09:00Z","timestamp":1790086140058,"version":"4.0.1"},"reference-count":20,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2021,2,15]],"date-time":"2021-02-15T00:00:00Z","timestamp":1613347200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Human papillomavirus (HPV) tests differ for technology, targets, and information on the genotype and viral load. In this study, we evaluated the performance of the Seegene Anyplex II HPV HR (Anyplex) assay in the detection of cervical intraepithelial lesions (CIN) and as a test\u2010of\u2010cure in the follow\u2010up after surgical treatment. One hundred and sixty\u2010seven women referred to the European Institute of Oncology, Milan, for surgical treatment of CIN2+ were enrolled. A cervical sample was taken before treatment and at the first follow\u2010up visit: on these samples, Qiagen Hybrid Capture 2 (HC2), Roche Linear Array HPV Test (Linear Array), cytology and histology were performed at baseline, HC2, and cytology at follow\u2010up. Anyplex genotyping HPV test was performed on a post aliquot from liquid\u2010based cytology specimens when available. The concordance between Anyplex and HC2 was 93.6% at baseline and 76.7% at follow\u2010up (3\u20139 months after treatment), respectively. The concordance between Anyplex and Linear Array was evaluable only at baseline (92.9%). No recurrence occurred in women without the persistence of the same genotype at follow\u2010up. Seven women relapsed: six had persistence of the same genotypes (five HPV16, one HPV33, and one HPV39), while one tested negative not only with Anyplex but also with HC2 for the persistence of low\u2010risk genotype infection (HPV73 only detected by Linear Array). Anyplex test represents a valid option for HPV detection and genotyping in order to stratify women at risk of high\u2010grade lesions at baseline and to monitor patients treated for CIN2+ lesions during follow\u2010up.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.26862","type":"journal-article","created":{"date-parts":[[2021,2,10]],"date-time":"2021-02-10T11:17:53Z","timestamp":1612955873000},"page":"6340-6346","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Anyplex II HPV test in detection and follow\u2010up after surgical treatment of CIN2+ lesions"],"prefix":"10.1002","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1838-0163","authenticated-orcid":false,"given":"Fabio","family":"Bottari","sequence":"first","affiliation":[{"name":"Division of Laboratory Medicine European Institute of Oncology IRCCS  Milan Italy"},{"name":"Department of Biomedical Sciences University of Sassari  Sassari 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