{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T13:21:40Z","timestamp":1783948900564,"version":"3.55.0"},"reference-count":27,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human papillomavirus (HPV) testing with cytology triage for cervical cancer screening has proven to be useful. It is considered that a significant percentage of HPV\u2010positive women followed by reflex cytology have had multiple\u2010type HPV infections rather than single\u2010type infections. However, the effects of multiple\u2010type infections on changes in the cytomorphology of exfoliated cervical cells have not been investigated. The aim of this study was to validate simple manual microdissection (MMD) maneuver and investigate the HPV infection status of single cells isolated from Papanicolaou (Pap) smears prepared from women with multiple\u2010type infections. Using cytology samples from 90 patients with abnormal Pap smear results, we evaluated the efficiency of the MMD procedure and determined the HPV infection status of single squamous intraepithelial lesion (SIL) cells microdissected from patients with multiple\u2010type infection. When\u00a0validating the MMD procedure, the HPV\u2010positive rate was 81.5% using 119 MMD samples from the Pap smear in 61 cases with single\u2010type infection. This MMD procedure was able to efficiently collect single cells. Of 119 MMD samples from 29 cases with multiple\u2010type infection, the HPV\u2010positive rate was 42.9%, and most (96.1%) MMD samples exhibited only one genotype. Our MMD maneuver successfully identified HPV genotypes using single cells isolated from cytology specimens. A majority of single SIL cells prepared from multiple\u2010type infection cases turned out to contain only one genotype. In the future, the MMD method could be applied while studying the relationship between the morphological changes exhibited by SIL cells on Pap smear and the infected HPV genotype.<\/jats:p>","DOI":"10.1002\/jmv.26888","type":"journal-article","created":{"date-parts":[[2021,2,18]],"date-time":"2021-02-18T12:08:03Z","timestamp":1613650083000},"page":"5084-5094","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Human papillomavirus infection status of single cells isolated from cervical cytology specimens by simple manual microdissection"],"prefix":"10.1002","volume":"93","author":[{"given":"Natsumi","family":"Noji","sequence":"first","affiliation":[{"name":"Department of Medical Technology Faculty of Health Sciences, Kyorin University  Mitaka\u2010shi Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5994-9670","authenticated-orcid":false,"given":"Kaori","family":"Okayama","sequence":"additional","affiliation":[{"name":"Department of Medical Technology Faculty of Health Science, Gunma Paz University  Takasaki\u2010shi Gunma Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mizue","family":"Oda","sequence":"additional","affiliation":[{"name":"Department of Gynecology Genki Plaza Medical Center for Health Care  Chiyoda\u2010ku Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsuyoshi","family":"Shimada","sequence":"additional","affiliation":[{"name":"Department of Medical Technology Faculty of Health Sciences, Kyorin University  Mitaka\u2010shi Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6152-3217","authenticated-orcid":false,"given":"Mitsuaki","family":"Okodo","sequence":"additional","affiliation":[{"name":"Department of Medical Technology Faculty of Health Sciences, Kyorin University  Mitaka\u2010shi Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,3]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/85.12.958"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.57.1.68"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.25630"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2009.01161.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.24323"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.25017"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.3970"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2008.05.002"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-015-1281-5"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.2144\/00291bm09"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.278.5342.1481"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.32919"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.25775"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/01913120500521018"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.5858\/2004-128-1372-MTFMTI"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v9.i6.1337"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1136\/mp.51.4.233"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1136\/jclinpath-2017-204561"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.2174\/1566524013364121"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.20599"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2009.05.006"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2008.00379.x"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers6042072"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/dji187"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-80-11-2931"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1086\/315086"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-8-28"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.26888","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jmv.26888","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.26888","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T07:24:47Z","timestamp":1692602687000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.26888"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3]]},"references-count":27,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2021,8]]}},"alternative-id":["10.1002\/jmv.26888"],"URL":"https:\/\/doi.org\/10.1002\/jmv.26888","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,3]]},"assertion":[{"value":"2021-01-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-02-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}