{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T14:13:46Z","timestamp":1782742426298,"version":"3.54.5"},"reference-count":34,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2021,3,25]],"date-time":"2021-03-25T00:00:00Z","timestamp":1616630400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81670560"],"award-info":[{"award-number":["81670560"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["18411966500"],"award-info":[{"award-number":["18411966500"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010098","name":"Shanghai Association for Science and Technology","doi-asserted-by":"publisher","award":["19YF1441200"],"award-info":[{"award-number":["19YF1441200"]}],"id":[{"id":"10.13039\/100010098","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study aimed to explore the value of baseline serum exosome\u2010derived miRNAs for predicting HBeAg seroconversion in chronic hepatitis B (CHB) patients treated with peginterferon (Peg\u2010IFN). A total of 120 treatment\u2010na\u00efve HBeAg\u2010positive CHB patients who received Peg\u2010IFN therapy (48 weeks) were enrolled. Next\u2010generation sequencing was performed to screen the serum exosomal miRNAs that were associated with Peg\u2010IFN treatment outcome, and qRT\u2010PCR was used to validate them. The area under the receiver operating characteristic curve (AUROC) was used to evaluate the predictive efficacy of biomarkers. Thirty\u2010three patients (27.5%) achieved HBeAg seroconversion (response group), and 87 patients (72.5%) did not achieve HBeAg seroconversion (nonresponse group). In the identification cohort, 40 serum exosome\u2010derived miRNAs were differentially expressed between the response group (four patients) and the nonresponse group (four patients). In the confirmation cohort, the expression levels of serum exosomal miR\u2010194\u20105p (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001) and miR\u201022\u20103p (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001) were significantly downregulated in the response group (29 patients) compared to the nonresponse group (83 patients). Multivariate analysis identified baseline serum exosomal miR\u2010194\u20105p, miR\u201022\u20103p, alanine aminotransferase (ALT), and HBV DNA as independent predictors of HBeAg seroconversion (all <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). The AUROCs of serum exosomal miRNAs (0.77 and 0.75 for miR\u2010194\u20105p and miR\u201022\u20103p, respectively) were higher than that of ALT (0.70) and HBV DNA (0.69). The combination of exosomal miR\u2010194\u20105p and miR\u201022\u20103p further improved the predictive performance with an AUROC of 0.82. Baseline serum exosomal miR\u2010194\u20105p and miR\u201022\u20103p may serve as novel biomarkers to predict HBeAg seroconversion in CHB patients treated with Peg\u2010IFN.<\/jats:p>","DOI":"10.1002\/jmv.26916","type":"journal-article","created":{"date-parts":[[2021,3,5]],"date-time":"2021-03-05T10:48:47Z","timestamp":1614941327000},"page":"4939-4948","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Baseline serum exosome\u2010derived miRNAs predict HBeAg seroconversion in chronic hepatitis B patients treated with peginterferon"],"prefix":"10.1002","volume":"93","author":[{"given":"Qiankun","family":"Hu","sequence":"first","affiliation":[{"name":"Department of Liver Diseases, Shanghai Public Health Clinical Center Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianqian","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Liver Diseases, 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