{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:12:13Z","timestamp":1772118733620,"version":"3.50.1"},"reference-count":16,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2021,4,23]],"date-time":"2021-04-23T00:00:00Z","timestamp":1619136000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["5T32LM012410"],"award-info":[{"award-number":["5T32LM012410"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Inpatient coronavirus disease 2019 (COVID\u201019) cases present enormous costs to patients and health systems in the United States. Many hospitalized patients may continue testing COVID\u201019 positive even after the resolution of symptoms. Thus, a pressing concern for clinicians is the safety of discharging these asymptomatic patients if they have any remaining infectivity. This case report explores the viral viability in a patient with persistent COVID\u201019 over the course of a 2\u2010month hospitalization. Positive nasopharyngeal swab samples were collected and isolated in the laboratory and analyzed by quantitative reverse\u2010transcription polymerase chain reactions (qRT\u2010PCR), and serology was tested for neutralizing antibodies throughout the hospitalization period. The patient experienced waning symptoms by hospital day 40 and had no viable virus growth by hospital day 41, suggesting no risk of infectivity, despite positive RT\u2010PCR results which prolonged his hospital stay. Notably, this case showed infectivity for at least 24 days after disease onset, which is longer than the discontinuation of transmission\u2010based precautions recommended by the Center for Disease Control and Prevention. Thus, our findings suggest that the timeline for discontinuing transmission\u2010based precautions may need to be extended for patients with severe and prolonged COVID\u201019 disease. Additional large\u2010scale studies are needed to draw definitive conclusions on the appropriate clinical management for these patients.<\/jats:p>\n                  <jats:p>\u200b<\/jats:p>","DOI":"10.1002\/jmv.27001","type":"journal-article","created":{"date-parts":[[2021,4,8]],"date-time":"2021-04-08T07:50:36Z","timestamp":1617868236000},"page":"4570-4575","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["SARS\u2010CoV\u20102 show no infectivity at later stages in a prolonged COVID\u201019 patient despite positivity in RNA testing"],"prefix":"10.1002","volume":"93","author":[{"given":"Xiu\u2010Feng","family":"Wan","sequence":"first","affiliation":[{"name":"MU Center for Influenza and Emerging Infectious Diseases University of Missouri  Columbia Missouri USA"},{"name":"Department of Molecular Microbiology and Immunology, School of Medicine University of Missouri  Columbia Missouri USA"},{"name":"Bond Life Sciences Center University of Missouri  Columbia Missouri USA"},{"name":"Department of Electrical Engineering &amp; Computer Science, College of Engineering University of Missouri  Columbia Missouri USA"},{"name":"MU Institute for Data Science and Informatics University of Missouri  Columbia Missouri USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5272-7170","authenticated-orcid":false,"given":"Cynthia Y.","family":"Tang","sequence":"additional","affiliation":[{"name":"MU Center for Influenza and Emerging Infectious Diseases University of Missouri  Columbia Missouri USA"},{"name":"Department of Molecular Microbiology and Immunology, School of Medicine University of Missouri  Columbia Missouri USA"},{"name":"Bond Life Sciences Center University of Missouri  Columbia Missouri USA"},{"name":"MU Institute for Data Science and Informatics University of Missouri  Columbia Missouri USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Detlef","family":"Ritter","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Missouri  Columbia Missouri USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Wang","sequence":"additional","affiliation":[{"name":"MU Center for Influenza and Emerging Infectious Diseases University of Missouri  Columbia Missouri USA"},{"name":"Department of Molecular Microbiology and Immunology, School of Medicine University of Missouri  Columbia Missouri USA"},{"name":"Bond Life Sciences Center University of Missouri  Columbia Missouri USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Li","sequence":"additional","affiliation":[{"name":"Viral Diseases Branch Walter Reed Army Institute of Research  Silver Spring Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen","family":"Segovia","sequence":"additional","affiliation":[{"name":"MU Center for Influenza and Emerging Infectious Diseases University of Missouri  Columbia Missouri USA"},{"name":"Department of Molecular Microbiology and Immunology, School of Medicine University of Missouri  Columbia Missouri USA"},{"name":"Bond Life Sciences Center University of Missouri  Columbia Missouri USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martina","family":"Kosikova","sequence":"additional","affiliation":[{"name":"Division of Viral Products, Office of Vaccines Research and Review, Center for Biologics Evaluation and Research US Food and Drug Administration  Silver Spring Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc","family":"Johnson","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology and Immunology, School of Medicine University of Missouri  Columbia Missouri USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyung J.","family":"Kwon","sequence":"additional","affiliation":[{"name":"Division of Viral Products, Office of Vaccines Research and Review, Center for Biologics Evaluation and Research US Food and Drug Administration  Silver Spring Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hang","family":"Xie","sequence":"additional","affiliation":[{"name":"Division of Viral Products, Office of Vaccines Research and Review, Center for Biologics Evaluation and Research US Food and Drug Administration  Silver Spring Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard D.","family":"Hammer","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Missouri  Columbia Missouri USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jane A.","family":"McElroy","sequence":"additional","affiliation":[{"name":"Department of Family Medicine, School of Medicine University of Missouri  Columbia Missouri USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aws","family":"Hamid","sequence":"additional","affiliation":[{"name":"Department of Radiology, School of Medicine University of Missouri  Columbia Missouri USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalie D.","family":"Collins","sequence":"additional","affiliation":[{"name":"Viral Diseases Branch Walter Reed Army Institute of Research  Silver Spring Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Hang","sequence":"additional","affiliation":[{"name":"Viral Diseases Branch Walter Reed Army Institute of Research  Silver Spring Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simone","family":"Camp","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Missouri  Columbia Missouri USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,4,23]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"World Health Organization.\u00a0Coronavirus Disease (COVID\u201019) Dashboard;\u00a02021.https:\/\/covid19.who.int\/. Accessed February 04 2021."},{"key":"e_1_2_8_3_1","volume-title":"Average cost of hospital care for COVID\u201019 ranges from $51,000 to $78,000, based on age","author":"Hackett M.","year":"2020"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.m3097"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.m1923"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-20568-4"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41420-020-00375-y"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa638"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1136\/bcr-2020-241087"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.614436"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-21111-9"},{"key":"e_1_2_8_12_1","unstructured":"CDC.\u00a0Discontinuation of transmission\u2010based\u00a0precautions and disposition of patients with COVID\u201019 in healthcare settings (interim guidance);\u00a02020"},{"key":"e_1_2_8_13_1","unstructured":"Royal College of Physicians.\u00a0National Early Warning Score (NEWS) 2;2017.https:\/\/www.rcplondon.ac.uk\/projects\/outputs\/national-early-warning-score-news-2"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.aje.a118408"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.25952"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.2471\/BLT.20.253591"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.idh.2020.05.002"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.27001","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jmv.27001","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.27001","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,9]],"date-time":"2024-07-09T01:41:22Z","timestamp":1720489282000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.27001"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4,23]]},"references-count":16,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2021,7]]}},"alternative-id":["10.1002\/jmv.27001"],"URL":"https:\/\/doi.org\/10.1002\/jmv.27001","archive":["Portico"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2021.03.18.21253228","asserted-by":"object"}]},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4,23]]},"assertion":[{"value":"2021-03-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-04-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-04-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}