{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T01:55:01Z","timestamp":1773798901495,"version":"3.50.1"},"reference-count":13,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2021,5,3]],"date-time":"2021-05-03T00:00:00Z","timestamp":1620000000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>The accurate laboratory detection of the\u00a0severe acute respiratory syndrome coronavirus 2 (SARS\u2010CoV\u20102) is a crucial element in the fight against coronavirus disease 2019 (COVID\u201019). Reverse transcription\u2010polymerase chain reaction testing on combined oral and nasopharyngeal swab (ONPS) suffers from several limitations, including the need for qualified personnel, the discomfort caused by invasive nasopharyngeal sample collection, and the possibility of swab and transport media shortage. Testing on saliva would represent an advancement. The aim of this study was to compare the concordance between saliva samples and ONPS for the detection of SARS\u2010CoV\u20102 on various commercial and laboratory\u2010developed tests (LDT). Individuals were recruited from eight institutions in Quebec, Canada, if they had SARS\u2010CoV\u20102 RNA detected on a recently collected ONPS, and accepted to provide another ONPS, paired with saliva. Assays available in the different laboratories (Abbott RealTime SARS\u2010CoV\u20102, Cobas\u00ae SARS\u2010CoV\u20102, Simplexa\u2122 COVID\u201019 Direct, Allplex\u2122 2019\u2010nCoV, RIDA\u00aeGENE SARS\u2010CoV\u20102, and an LDT preceded by three different extraction methods) were used to determine the concordance between saliva and ONPS results. Overall, 320 tests were run from a total of 125 saliva and ONPS sample pairs. All assays yielded similar sensitivity when saliva was compared to ONPS, with the exception of one LDT (67% vs. 93%). The mean difference in cycle threshold (\u2206<jats:italic>C<\/jats:italic>\n<jats:sub>t<\/jats:sub>) was generally (but not significantly) in favor of the ONPS for all nucleic acid amplification tests. The maximum mean \u2206\u200b\u200b\u200b\u200b\u200b<jats:italic>C<\/jats:italic>\n<jats:sub>t<\/jats:sub> was 2.0, while individual \u2206<jats:italic>C<\/jats:italic>\n<jats:sub>t<\/jats:sub> varied importantly from \u221217.5 to 12.4. Saliva seems to be associated with sensitivity similar to ONPS for the detection of SARS\u2010CoV\u20102 by various assays.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.27026","type":"journal-article","created":{"date-parts":[[2021,4,15]],"date-time":"2021-04-15T00:48:56Z","timestamp":1618447736000},"page":"5333-5338","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Comparison of saliva with oral and nasopharyngeal swabs for SARS\u2010CoV\u20102 detection on various commercial and laboratory\u2010developed assays"],"prefix":"10.1002","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8936-3307","authenticated-orcid":false,"given":"Annie\u2010Claude","family":"Labb\u00e9","sequence":"first","affiliation":[{"name":"Service de maladies infectieuses et de microbiologie, D\u00e9partement de m\u00e9decine sp\u00e9cialis\u00e9e H\u00f4pital Maisonneuve\u2010Rosemont \u2010 CIUSSS de l'Est\u2010de\u2010l'Ile\u2010de\u2010Montr\u00e9al  Montr\u00e9al Qu\u00e9bec Canada"},{"name":"D\u00e9partement de microbiologie, infectiologie et immunologie Universit\u00e9 de Montr\u00e9al  Montr\u00e9al Qu\u00e9bec Canada"},{"name":"D\u00e9partement clinique de m\u00e9decine de laboratoire Centre Hospitalier de l'Universit\u00e9 de Montr\u00e9al  Montr\u00e9al Qu\u00e9bec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick","family":"Benoit","sequence":"additional","affiliation":[{"name":"D\u00e9partement de microbiologie, infectiologie et immunologie Universit\u00e9 de Montr\u00e9al  Montr\u00e9al Qu\u00e9bec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah","family":"Gobeille Par\u00e9","sequence":"additional","affiliation":[{"name":"Service de microbiologie\u2010infectiologie Centre Hospitalier Universitaire de Qu\u00e9bec \u2013 Universit\u00e9 Laval  Qu\u00e9bec Qu\u00e9bec 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