{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T23:40:30Z","timestamp":1790725230862,"version":"4.1.0"},"reference-count":26,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2021,5,19]],"date-time":"2021-05-19T00:00:00Z","timestamp":1621382400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>As severe acute respiratory syndrome coronavirus\u20102 (SARS\u2010CoV\u20102) infections continue, there is a substantial need for cost\u2010effective and large\u2010scale testing that utilizes specimens that can be readily collected from both symptomatic and asymptomatic individuals in various community settings. Although multiple diagnostic methods utilize nasopharyngeal specimens, saliva specimens represent an attractive alternative as they can rapidly and safely be collected from different populations. While saliva has been described as an acceptable clinical matrix for the detection of SARS\u2010CoV\u20102, evaluations of analytic performance across platforms for this specimen type are limited. Here, we used a novel sensitive RT\u2010PCR\/MALDI\u2010TOF mass spectrometry\u2010based assay (Agena MassARRAY\u00ae) to detect SARS\u2010CoV\u20102 in saliva specimens. The platform demonstrated high diagnostic sensitivity and specificity when compared to matched patient upper respiratory specimens. We also evaluated the analytical sensitivity of the platform and determined the limit of detection of the assay to be 1562.5 copies\/ml. Furthermore, across the five individual target components of this assay, there was a range in analytic sensitivities for each target with the N2 target being the most sensitive. Overall, this system also demonstrated comparable performance when compared to the detection of SARS\u2010CoV\u20102 RNA in saliva by the cobas\u00ae 6800\/8800 SARS\u2010CoV\u20102 real\u2010time RT\u2010PCR Test (Roche). Together, we demonstrate that saliva represents an appropriate matrix for SARS\u2010CoV\u20102 detection on the novel Agena system as well as on a conventional real\u2010time RT\u2010PCR assay. We conclude that the MassARRAY\u00ae system is a sensitive and reliable platform for SARS\u2010CoV\u20102 detection in saliva, offering scalable throughput in a large variety of clinical laboratory settings.<\/jats:p>","DOI":"10.1002\/jmv.27069","type":"journal-article","created":{"date-parts":[[2021,5,9]],"date-time":"2021-05-09T02:38:34Z","timestamp":1620527914000},"page":"5481-5486","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["RT\u2010PCR\/MALDI\u2010TOF mass spectrometry\u2010based detection of SARS\u2010CoV\u20102 in saliva specimens"],"prefix":"10.1002","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6217-7264","authenticated-orcid":false,"given":"Matthew M.","family":"Hernandez","sequence":"first","affiliation":[{"name":"Department of Pathology, Molecular, and Cell\u2010Based Medicine Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Radhika","family":"Banu","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory, Department of Pathology, Molecular, and Cell\u2010Based Medicine Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paras","family":"Shrestha","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory, Department of Pathology, Molecular, and Cell\u2010Based Medicine Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Armi","family":"Patel","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory, Department of Pathology, Molecular, and Cell\u2010Based Medicine Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Chen","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory, Department of Pathology, Molecular, and Cell\u2010Based Medicine Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liyong","family":"Cao","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory, Department of Pathology, Molecular, and Cell\u2010Based Medicine Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shelcie","family":"Fabre","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory, Department of Pathology, Molecular, and Cell\u2010Based Medicine Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Microbiology Icahn School of Medicine at Mount Sinai  New York New York USA"},{"name":"The Graduate School of Biomedical Sciences Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heidi","family":"Lopez","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory, Department of Pathology, Molecular, and Cell\u2010Based Medicine Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Numthip","family":"Chiu","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory, Department of Pathology, Molecular, and Cell\u2010Based Medicine Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biana","family":"Shifrin","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory, Department of Pathology, Molecular, and Cell\u2010Based Medicine Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inessa","family":"Zapolskaya","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory, Department of Pathology, Molecular, and Cell\u2010Based Medicine Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa","family":"Flores","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory, Department of Pathology, Molecular, and Cell\u2010Based Medicine Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pui Yiu","family":"Lee","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory, Department of Pathology, Molecular, and Cell\u2010Based Medicine Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Casta\u00f1eda","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda, Facultad de Ciencias Naturales, Grupo de Investigaciones Microbiol\u00f3gicas\u2010UR (GIMUR) Universidad del Rosario  Bogot\u00e1 Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1344-9312","authenticated-orcid":false,"given":"Juan David","family":"Ram\u00edrez","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda, Facultad de Ciencias Naturales, Grupo de Investigaciones Microbiol\u00f3gicas\u2010UR (GIMUR) Universidad del Rosario  Bogot\u00e1 Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey","family":"Jhang","sequence":"additional","affiliation":[{"name":"Department of Pathology, Molecular, and Cell\u2010Based Medicine Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuliana","family":"Osorio","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory, Department of Pathology, Molecular, and Cell\u2010Based Medicine Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6960-0763","authenticated-orcid":false,"given":"Melissa R.","family":"Gitman","sequence":"additional","affiliation":[{"name":"Department of Pathology, Molecular, and Cell\u2010Based Medicine Icahn School of Medicine at Mount Sinai  New York New York USA"},{"name":"Clinical Microbiology Laboratory, Department of Pathology, Molecular, and Cell\u2010Based Medicine Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1917-0785","authenticated-orcid":false,"given":"Michael D.","family":"Nowak","sequence":"additional","affiliation":[{"name":"Department of Pathology, Molecular, and Cell\u2010Based Medicine Icahn School of Medicine at Mount Sinai  New York New York USA"},{"name":"Clinical Microbiology Laboratory, Department of Pathology, Molecular, and Cell\u2010Based Medicine Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David L.","family":"Reich","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Perioperative and Pain Medicine Icahn School of Medicine at Mount Sinai  New York New York 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