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However, very few studies explore the susceptibility of animals to severe acute respiratory syndrome coronavirus 2 (SARS\u2010CoV\u20102). Viral attachment as a crucial step for cross\u2010species infection requires angiotensin\u2010converting enzyme 2 (ACE2) as a receptor and depends on TMPRSS2 protease activity. Here, we searched the genomes of metazoans from different classes using an extensive BLASTP survey and found ACE2 and TMPRSS2 occur in vertebrates, but some vertebrates lack Tmprss2. We identified 6 amino acids among 25 known human ACE2 residues are highly associated with the binding of ACE2 to SARS\u2010CoV\u20102 (<jats:italic>p<\/jats:italic> value\u2009&lt;\u2009.01) by Fisher exact test, and following this, calculated the probability of viral attachment within each species by the randomForest function from R randomForest library. Furthermore, we observed that Ace2 selected from seven animals based on the above analysis lack the hydrophobic contacts identified on human ACE2, indicating less affinity of SARS\u2010CoV\u20102 to Ace2 in animals than humans. Finally, the alignment of 3D structure between human ACE2 and other animals by I\u2010TASSER and TM\u2010align displayed a reasonable structure for viral attachment within these species. Taken together, our data may shed light on the human\u2010to\u2010animal transmission of SARS\u2010CoV\u20102.<\/jats:p>","DOI":"10.1002\/jmv.27073","type":"journal-article","created":{"date-parts":[[2021,5,11]],"date-time":"2021-05-11T18:06:30Z","timestamp":1620756390000},"page":"5487-5504","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Comparative analyses of ACE2 and TMPRSS2 gene: Implications for the risk to which vertebrate animals are susceptible to SARS\u2010CoV\u20102"],"prefix":"10.1002","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8631-3654","authenticated-orcid":false,"given":"Chen","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Veterinary Science University of Kentucky  Lexington Kentucky USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Jiang","sequence":"additional","affiliation":[{"name":"Division of Epidemiology, Biostatistics, and Environmental Health, School of Public Health University of Memphis  Memphis Tennessee USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8362-4086","authenticated-orcid":false,"given":"Jie","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Diagnosis, School of Medicine, Cheeloo College of Medicine Shandong University  Jinan Shandong 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