{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:56:12Z","timestamp":1789692972867,"version":"4.0.1"},"reference-count":18,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2021,6,6]],"date-time":"2021-06-06T00:00:00Z","timestamp":1622937600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2021,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Rapid diagnostics for severe acute respiratory syndrome coronavirus 2 (SARS\u2010CoV\u20102) are paramount for\u00a0reducing\u00a0the spread of the current pandemic. During additional seasonal epidemics with influenza A\/B and respiratory syncytial virus (RSV), the clinical signs and symptoms cannot be distinguished easily from SARS\u2010CoV\u20102. Therefore, a new assay combining four targets in the form of the new Xpert Xpress SARS\u2010CoV\u20102\/Flu\/RSV assay was evaluated. The assay was compared to the Xpert Xpress SARS\u2010CoV\u20102, Xpert Xpress Flu\/RSV, Seegene Flu\/RSV, influenza A\/B r\u2010gene\u00ae and RSV\/hMPV r\u2010gene\u00ae. A total of 295 nasopharyngeal and throat swabs were tested at four institutes throughout Europe including 72 samples positive for SARS\u2010CoV\u20102, 65 for influenza A, 47 for influenza B, and 77 for RSV. The sensitivity of the new assay was above 95% for all targets, with the highest for SARS\u2010CoV\u20102 (97.2%). The overall correlation of SARS\u2010CoV\u20102 Ct values between Xpert Xpress SARS\u2010CoV\u20102 assay and Xpert Xpress SARS\u2010CoV\u20102\/Flu\/RSV assay was high. The agreement between Ct values above 30 showed the multiplex giving higher Ct values for SARS\u2010CoV\u20102 on average than the singleplex assay. In conclusion, the new assay is a rapid and reliable alternative with less hands\u2010on time for the detection of not one, but four upper respiratory tract pathogens that may circulate at the same time.<\/jats:p>","DOI":"10.1002\/jmv.27111","type":"journal-article","created":{"date-parts":[[2021,5,31]],"date-time":"2021-05-31T11:09:26Z","timestamp":1622459366000},"page":"5798-5804","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["European multicenter evaluation of Xpert\u00ae Xpress SARS\u2010CoV\u20102\/Flu\/RSV test"],"prefix":"10.1002","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6455-9802","authenticated-orcid":false,"given":"Femke","family":"Wolters","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology Radboud University Medical Center Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Gr\u00fcnberg","sequence":"additional","affiliation":[{"name":"Institute of Medical Virology University of Zurich Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Huber","sequence":"additional","affiliation":[{"name":"Institute of Medical Virology University of Zurich Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harald H.","family":"Kessler","sequence":"additional","affiliation":[{"name":"Molecular Diagnostics Laboratory, Diagnostic and Research Institute of Hygiene, Microbiology, and Environmental Medicine Medical University of Graz Graz Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian","family":"Pr\u00fcller","sequence":"additional","affiliation":[{"name":"Clinical Institute of Medical and Laboratory Diagnostics Medical University of Graz Graz Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lanja","family":"Saleh","sequence":"additional","affiliation":[{"name":"Institute of Clinical Chemistry University Hospital of Zurich, University of Zurich Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"F\u00e9breau","sequence":"additional","affiliation":[{"name":"Department of Virology University Hospital of Rennes Rennes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0120-7962","authenticated-orcid":false,"given":"Janette","family":"Rahamat\u2010Langendoen","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Radboud University Medical Center Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Thibault","sequence":"additional","affiliation":[{"name":"Department of Virology University Hospital of Rennes Rennes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5446-2230","authenticated-orcid":false,"given":"Willem J. 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