{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T11:07:12Z","timestamp":1773659232663,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2021,6,12]],"date-time":"2021-06-12T00:00:00Z","timestamp":1623456000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2021,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>Geographical variation in severe acute respiratory syndrome coronavirus 2 (SARS\u00ad\u2010CoV\u00ad\u20102) spread requires seroprevalence studies based on local tests, but robust validation is needed. We summarize an evaluation of antibody tests used in a serological study of SARS\u2010\u00adCoV\u00ad\u20102 in Saint Petersburg, Russia. We validated three different antibody assays: chemiluminescent microparticle immunoassay (CMIA) Abbott Architect SARS\u2010\u00adCoV\u00ad\u20102 immunoglobulin G (IgG), enzyme\u00ad linked immunosorbent assay (ELISA) CoronaPass total antibodies test, and ELISA SARS\u00ad\u2010CoV\u2010\u00ad2\u2010\u00adIgG\u2010\u00adEIA\u2010\u00adBEST. Clinical sensitivity was estimated with the SARS\u00ad\u2010CoV\u00ad\u20102 polymerase chain reaction (PCR) test as the gold standard using manufacturer recommended cutoff. Specificity was estimated using pre\u00adpandemic sera samples. The median time between positive PCR test results and antibody tests was 21 weeks. Measures of concordance were calculated against the microneutralization test (MNA).Sensitivity was equal to 91.1% (95% confidence intervbal [CI]: 78.8\u201397.5), 90% (95% CI: 76.4\u201396.4), and 63.1% (95% CI [50.2\u201374.7]) for ELISA Coronapass, ELISA Vector\u00adBest, and CMIA Abbott, respectively. Specificity was equal to 100% for all the tests. Comparison of receiver operating characteristics has shown lower AUC for CMIA Abbott. The cut\u00adoff SC\/O ratio of 0.28 for CMIA Abbott resulted in a sensitivity of 80% at the same level of specificity. Less than 33% of the participants with positive antibody test results had neutralizing antibodies in titers 1:80 and above. Antibody assays results and MNA correlated moderately. This study encourages the use of local antibody tests and sets the reference for seroprevalence correction. Available tests' sensitivity allows detecting antibodies within the majority of PCR\u00ad positive individuals. The Abbott assay sensitivity can be improved by incorporating a new cut\u00adoff. Manufacturers' test characteristics may introduce bias into the study results.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jmv.27126","type":"journal-article","created":{"date-parts":[[2021,6,3]],"date-time":"2021-06-03T16:12:32Z","timestamp":1622736752000},"page":"5846-5852","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Evaluation of the performance of SARS\u2010\u00adCoV\u00ad\u20102 antibody assays for a longitudinal population\u00adbased study of COVID\u2010\u00ad19 spread in St. Petersburg, Russia"],"prefix":"10.1002","volume":"93","author":[{"given":"Anton","family":"Barchuk","sequence":"first","affiliation":[{"name":"Institute for Interdisciplinary Health Research (I2HR) European University at St. Petersburg  St. Petersburg Russia"},{"name":"NN Petrov National Research Medical Center of Oncology  St. Petersburg Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniil","family":"Shirokov","sequence":"additional","affiliation":[{"name":"Clinic \u201cScandinavia\u201d (LLC Ava\u00adPeter)  St. Petersburg Russia"},{"name":"Publication Activity Development Center ITMO University  St. Petersburg Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariia","family":"Sergeeva","sequence":"additional","affiliation":[{"name":"Department of Vaccinology Smorodintsev Research Institute of Influenza  St. Petersburg Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rustam","family":"Tursun\u00adzade","sequence":"additional","affiliation":[{"name":"Institute for Interdisciplinary Health Research (I2HR) European University at St. Petersburg  St. Petersburg Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4735-5792","authenticated-orcid":false,"given":"Olga","family":"Dudkina","sequence":"additional","affiliation":[{"name":"Institute for Interdisciplinary Health Research (I2HR) European University at St. Petersburg  St. Petersburg Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Varvara","family":"Tychkova","sequence":"additional","affiliation":[{"name":"Department of Etiology and Epidemiology Smorodintsev Research Institute of Influenza  Saint Petersburg Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lubov","family":"Barabanova","sequence":"additional","affiliation":[{"name":"Clinic \u201cScandinavia\u201d (LLC Ava\u00adPeter)  St. Petersburg Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dmitriy","family":"Skougarevskiy","sequence":"additional","affiliation":[{"name":"Institute for the Rule of Law European University at Saint Petersburg  Saint Petersburg Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daria","family":"Danilenko","sequence":"additional","affiliation":[{"name":"Department of Etiology and Epidemiology Smorodintsev Research Institute of Influenza  Saint Petersburg Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,6,12]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"1","article-title":"The paramount importance of serological surveys of SARS\u00adCoV\u00ad2 infection and immunity","author":"Goudsmit J.","year":"2020","journal-title":"Eur J Epidemiol"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2026116"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)31483-5"},{"key":"e_1_2_9_5_1","article-title":"Estimating COVID\u00ad19 antibody seroprevalence in Santa Clara County, California. 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