{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T19:17:12Z","timestamp":1771269432529,"version":"3.50.1"},"reference-count":15,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2021,7,6]],"date-time":"2021-07-06T00:00:00Z","timestamp":1625529600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2021,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Problem<\/jats:title><jats:p>The utility of the polymerase chain reaction (PCR) cycle threshold (<jats:italic>C<\/jats:italic><jats:sub>t<\/jats:sub>) values in the management of patients with coronavirus disease 2019 (COVID\u201019) remains controversial.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We assessed the correlation of severe acute respiratory syndrome coronavirus 2 (SARS\u2010CoV\u20102) <jats:italic>C<\/jats:italic><jats:sub>t<\/jats:sub> values in nasopharyngeal swab samples with the oxygen requirements at the time of sample collection. Specimens were tested with the Simplexa PCR platform, which targets the SARS\u2010CoV\u20102 <jats:italic>ORF1ab<\/jats:italic> and <jats:italic>S<\/jats:italic> genes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We identified 23 COVID\u201019 patients with 49 <jats:italic>C<\/jats:italic><jats:sub>t<\/jats:sub> values available. While <jats:italic>C<\/jats:italic><jats:sub>t<\/jats:sub> values from <jats:italic>ORF1ab<\/jats:italic> and <jats:italic>S<\/jats:italic> genes were highly correlated for a given specimen, there was no correlation between <jats:italic>C<\/jats:italic><jats:sub>t<\/jats:sub> values for any of these target genes and the oxygen requirements of the patient at the time of sample collection. We found no differences in the initial nor the nadir <jats:italic>C<\/jats:italic><jats:sub>t<\/jats:sub> values between survivors and non\u2010survivors or mild\/moderate versus severe\/critical illness at the maximum point of illness.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>SARS\u2010CoV\u20102 <jats:italic>C<\/jats:italic><jats:sub>t<\/jats:sub> values have limited value in the management of COVID\u201019.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.27171","type":"journal-article","created":{"date-parts":[[2021,7,2]],"date-time":"2021-07-02T04:10:17Z","timestamp":1625199017000},"page":"6059-6062","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Lack of correlation between the SARS\u2010CoV\u20102 cycle threshold (<i>C<\/i><sub>t<\/sub>) value and clinical outcomes in patients with COVID\u201019"],"prefix":"10.1002","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9584-5011","authenticated-orcid":false,"given":"Jose F.","family":"Camargo","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases University of Miami Miller School of Medicine  Miami Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6226-0375","authenticated-orcid":false,"given":"Rick Y.","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Miami Miller School of Medicine  Miami Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krishna V.","family":"Komanduri","sequence":"additional","affiliation":[{"name":"Division of Transplantation and Cellular Therapy Sylvester Comprehensive Cancer Center  Miami Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,7,6]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa1616"},{"key":"e_1_2_9_3_1","article-title":"Viral dynamics and immune correlates of COVID\u201019 disease severity","author":"Young BE","year":"2020","journal-title":"Clin Infect Dis"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa638"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-020-03913-9"},{"key":"e_1_2_9_6_1","unstructured":"Infectious Diseases Society of America. IDSA and AMP joint statement on the use of SARS\u2010CoV\u20102 PCR cycle threshold (Ct) values for clinical decision\u2010making. 2021;https:\/\/www.idsociety.org\/globalassets\/idsa\/public-health\/covid-19\/idsa-amp-statement.pdf"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa1199"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa851"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2020.09.007"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3346\/jkms.2021.36.e83"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-020-0869-5"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(20)30196-1"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamainternmed.2020.3862"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1136\/thoraxjnl-2020-215042"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1017\/ice.2020.1371"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.7326\/M20-6976"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.27171","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jmv.27171","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.27171","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T22:57:18Z","timestamp":1693004238000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.27171"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7,6]]},"references-count":15,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2021,10]]}},"alternative-id":["10.1002\/jmv.27171"],"URL":"https:\/\/doi.org\/10.1002\/jmv.27171","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,7,6]]},"assertion":[{"value":"2021-06-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-06-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-07-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}