{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:47:19Z","timestamp":1786978039495,"version":"build-2736575974"},"reference-count":10,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2021,8,5]],"date-time":"2021-08-05T00:00:00Z","timestamp":1628121600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Gargle samples have been proposed as a noninvasive method for detection of SARS\u2010CoV\u20102 RNA. The clinical performance of gargle specimens diluted in Cobas\u00ae PCR Media and in Cobas\u00ae Omni Lysis Reagent was compared to oropharyngeal\/nasopharyngeal swab (ONPS) for the detection of SARS\u2010CoV\u20102 RNA.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study Design<\/jats:title>\n                    <jats:p>Participants were recruited prospectively in two COVID\u201019 screening clinics. In addition to the ONPS, participants gargled with 5\u2009ml of natural spring water split in the laboratory as follows: 1\u2009ml was added to 4.3\u2009ml of polymerase chain reaction (PCR) media and 400\u2009\u03bcl was added to 200\u2009\u03bcl of lysis buffer. Testing was performed with the Cobas\u00ae SARS\u2010CoV\u20102 test on the Cobas\u00ae 6800 or 8800 platforms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Overall, 134\/647 (20.7%) participants were considered infected because the ONPS or at least one gargle test was positive. ONPS had, respectively, a sensitivity of 96.3% (95% confidence interval [CI]: 91.3\u201398.5); both gargle processing methods were slightly less but equally sensitive (90.3% [95% CI: 83.9\u201394.3]). When ONPS and gargle specimens were both positive, the mean cycle threshold (\n                      <jats:italic>C<\/jats:italic>\n                      <jats:sub>t<\/jats:sub>\n                      ) was significantly higher for gargles, suggesting lower viral loads.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Gargle specimens directly added in PCR Media provide a similar clinical sensitivity to chemical lysis, both having a slightly, not significantly, lower sensitivity to ONPS.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jmv.27245","type":"journal-article","created":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T09:49:16Z","timestamp":1627552156000},"page":"6837-6840","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Comparison of SARS\u2010CoV\u20102 detection with the Cobas\u00ae 6800\/8800 system on gargle samples using two sample processing methods with combined oropharyngeal\/nasopharyngeal swab"],"prefix":"10.1002","volume":"93","author":[{"given":"Patrick","family":"Benoit","sequence":"first","affiliation":[{"name":"D\u00e9partement de microbiologie, Infectiologie et Immunologie Universit\u00e9 de Montr\u00e9al Qu\u00e9bec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annie\u2010Claude","family":"Labb\u00e9","sequence":"additional","affiliation":[{"name":"D\u00e9partement de microbiologie, Infectiologie et Immunologie Universit\u00e9 de Montr\u00e9al Qu\u00e9bec Canada"},{"name":"Service d'infectiologie, D\u00e9partement de m\u00e9decine H\u00f4pital Maisonneuve\u2010Rosemont\u2014CIUSSS de l'Est\u2010de\u2010l'\u00cele\u2010de\u2010Montr\u00e9al, Montr\u00e9al Qu\u00e9bec 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Institut national de sant\u00e9 publique du Qu\u00e9bec Ste\u2010Anne\u2010de\u2010Bellevue Qu\u00e9bec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judith","family":"Fafard","sequence":"additional","affiliation":[{"name":"Laboratoire de sant\u00e9 publique du Qu\u00e9bec, Institut national de sant\u00e9 publique du Qu\u00e9bec Ste\u2010Anne\u2010de\u2010Bellevue Qu\u00e9bec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeannot","family":"Dumaresq","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Microbiologie et d'Infectiologie CISSS de Chaudi\u00e8re\u2010Appalaches, L\u00e9vis Qu\u00e9bec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phanie","family":"Beauchemin","sequence":"additional","affiliation":[{"name":"Service d'infectiologie, D\u00e9partement de m\u00e9decine H\u00f4pital Maisonneuve\u2010Rosemont\u2014CIUSSS de l'Est\u2010de\u2010l'\u00cele\u2010de\u2010Montr\u00e9al, 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