{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T05:52:51Z","timestamp":1768715571235,"version":"3.49.0"},"reference-count":14,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2021,8,7]],"date-time":"2021-08-07T00:00:00Z","timestamp":1628294400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We aimed to compare reverse transcription\u2010polymerase chain reaction (RT\u2010PCR) results of nasopharyngeal aspiration (NA) and nasopharyngeal swab (NS) samples in the diagnosis of coronavirus disease 2019. NS was obtained with a dacron swab and NA was performed by aspiration cannula. The sampling was performed by an otolaryngologist to ensure standardized correct sampling from the nasopharynx. RT\u2010PCR was performed for the detection of severe acute respiratory syndrome coronavirus 2 (SARS\u2010CoV\u20102). The level of agreement between the result of NA and NS samples for each patient was analyzed. The Ct values were compared. Thirty\u2010three patients were enrolled in the study with a mean age of 56.3 years. Thirteen subjects resulted negative with both NS and NA; 20 subjects resulted positive with NA and 18 subjects resulted positive with NS. The mean values of Ct for NA samples and NS samples were 24.6\u2009\u00b1\u20095.9 and 24\u2009\u00b1\u20096.7, respectively. There was no statistical difference between Ct values of NA and NS samples (<jats:italic>p<\/jats:italic>\u2009=\u20090.48). RT\u2010PCR for SARS\u2010Cov2 performed with NA sample and NS sample showed a strong correlation regarding the positivity\/negativity and the Ct values.<\/jats:p>","DOI":"10.1002\/jmv.27250","type":"journal-article","created":{"date-parts":[[2021,7,31]],"date-time":"2021-07-31T14:17:50Z","timestamp":1627741070000},"page":"6693-6695","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Comparison of nasopharyngeal swab and nasopharyngeal aspiration in adults for SARS\u2010CoV\u20102 identification using reverse transcription\u2010polymerase chain reaction"],"prefix":"10.1002","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7108-9194","authenticated-orcid":false,"given":"Selin","family":"\u00dcnsaler","sequence":"first","affiliation":[{"name":"Department of Otolaryngology Ko\u00e7 University School of Medicine  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ay\u015fe","family":"Okan","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Division of Molecular Microbiology Ko\u00e7 University Hospital  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S\u00fcda","family":"Tekin","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology and Infectious Diseases Ko\u00e7 University School of Medicine  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ay\u015fenur M.","family":"Haf\u0131z","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Ko\u00e7 University School of Medicine  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ozan","family":"G\u00f6kler","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Ko\u00e7 University School of Medicine  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ozan","family":"Altunta\u015f","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Ko\u00e7 University School of Medicine  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,8,7]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2020.25.3.2000045"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/CCE.0000000000000154"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/pai.13366"},{"issue":"1","key":"e_1_2_7_5_1","first-page":"23","article-title":"Upper respiratory tract sampling in COVID\u201019","volume":"42","author":"Mawaddah A","year":"2020","journal-title":"Malays J Pathol"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2020.1745095"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa760"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.5811\/westjem.2020.4.47370"},{"issue":"18","key":"e_1_2_7_9_1","first-page":"1843","article-title":"Detection of SARS\u2010CoV\u20102 in different types of clinical specimens","volume":"323","author":"Wang W","year":"2020","journal-title":"JAMA"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa722"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.7326\/M20-1495"},{"key":"e_1_2_7_12_1","doi-asserted-by":"crossref","unstructured":"NazerianP SaccoRM SolbiatiM et al. Laryngotracheal aspiration test reduce the false negative rate in patients with suspected SARS\u2010COV\u20102 pneumonia despite a negative nasopharyngeal swab.Eur J Intern Med. Published online June 24 2021.https:\/\/doi.org\/10.1016\/j.ejim.2021.06.019","DOI":"10.1016\/j.ejim.2021.06.019"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2020.115228"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.11.4337-4339.2002"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01209-08"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.27250","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jmv.27250","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.27250","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T23:21:41Z","timestamp":1693005701000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.27250"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,8,7]]},"references-count":14,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["10.1002\/jmv.27250"],"URL":"https:\/\/doi.org\/10.1002\/jmv.27250","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,8,7]]},"assertion":[{"value":"2021-06-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-07-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-08-07","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}