{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T10:57:09Z","timestamp":1791889029375,"version":"4.3.4"},"reference-count":15,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T00:00:00Z","timestamp":1630454400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The current study aimed at characterizing the dynamics of SARS\u2010CoV\u20102 nucleocapsid (N) antigenemia in a cohort of critically ill adult COVID\u201019 patients and assessing its potential association with plasma levels of biomarkers of clinical severity and mortality. Seventy\u2010three consecutive critically ill COVID\u201019 patients (median age, 65 years) were recruited. Serial plasma (<jats:italic>n<\/jats:italic>\u2009=\u2009340) specimens were collected. A lateral flow immunochromatography assay and reverse\u2010transcription polymerase chain reaction (RT\u2010PCR) were used for SARS\u2010CoV\u20102 N protein detection and RNA quantitation and in plasma, respectively. Serum levels of inflammatory and tissue\u2010damage biomarkers in paired specimens were measured. SARS\u2010CoV\u2010RNA N\u2010antigenemia and viral RNAemia were documented in 40.1% and 35.6% of patients, respectively at a median of 9 days since\u00a0symptoms onset. The level of agreement between the qualitative results returned by the N\u2010antigenemia assay and plasma RT\u2010PCR was moderate (<jats:italic>k\u2009<\/jats:italic>=\u20090.57; <jats:italic>p\u2009<\/jats:italic>&lt;\u20090.0001). A trend towards higher SARS\u2010CoV\u20102 RNA loads was seen in plasma specimens testing positive for N\u2010antigenemia assay than in those yielding negative results (<jats:italic>p\u2009<\/jats:italic>=\u20090.083). SARS\u2010CoV\u20102 RNA load in tracheal aspirates was significantly higher (<jats:italic>p\u2009<\/jats:italic>&lt;\u20090.001) in the presence of concomitant N\u2010antigenemia than in its absence. Significantly higher serum levels of ferritin, lactose dehydrogenase, C\u2010reactive protein, and D\u2010dimer were quantified in paired plasma SARS\u2010CoV\u20102 N\u2010positive specimens than in those testing negative. Occurrence of SARS\u2010CoV\u20102 N\u2010antigenemia was not associated with increased mortality in univariate logistic regression analysis (odds ratio, 1.29; 95% confidence interval, 0.49\u20103.34; <jats:italic>p\u2009<\/jats:italic>=\u20090.59). In conclusion, SARS\u2010CoV\u20102 N\u2010antigenemia detection is relatively common in ICU patients and appears to associate with increased serum levels of inflammation and tissue\u2010damage markers. Whether this virological parameter may behave as a biomarker of poor clinical outcome awaits further investigations.<\/jats:p>","DOI":"10.1002\/jmv.27300","type":"journal-article","created":{"date-parts":[[2021,8,27]],"date-time":"2021-08-27T16:27:29Z","timestamp":1630081649000},"page":"222-228","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["SARS\u2010CoV\u20102 N\u2010antigenemia in critically ill adult COVID\u201019 patients: Frequency and association with inflammatory and tissue\u2010damage biomarkers"],"prefix":"10.1002","volume":"94","author":[{"given":"Beatriz","family":"Olea","sequence":"first","affiliation":[{"name":"Microbiology Service Clinic University Hospital, INCLIVA Health Research Institute  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eliseo","family":"Albert","sequence":"additional","affiliation":[{"name":"Microbiology Service Clinic University Hospital, INCLIVA Health Research Institute  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignacio","family":"Torres","sequence":"additional","affiliation":[{"name":"Microbiology Service Clinic University Hospital, INCLIVA Health Research Institute  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Gozalbo\u2010Rovira","sequence":"additional","affiliation":[{"name":"Department of Microbiology, School of Medicine University of Valencia  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nieves","family":"Carbonell","sequence":"additional","affiliation":[{"name":"Medical Intensive Care Unit Clinic University Hospital, INCLIVA Health Research Institute  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"Ferreres","sequence":"additional","affiliation":[{"name":"Medical Intensive Care Unit Clinic University Hospital, INCLIVA Health Research Institute  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandrine","family":"Poujois","sequence":"additional","affiliation":[{"name":"Microbiology Service Clinic University Hospital, INCLIVA Health Research Institute  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa","family":"Costa","sequence":"additional","affiliation":[{"name":"Microbiology Service Clinic University Hospital, INCLIVA Health Research Institute  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Colomina","sequence":"additional","affiliation":[{"name":"Microbiology Service Clinic University Hospital, INCLIVA Health Research Institute  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jes\u00fas","family":"Rodr\u00edguez\u2010D\u00edaz","sequence":"additional","affiliation":[{"name":"Department of Microbiology, School of Medicine University of Valencia  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda L.","family":"Blasco","sequence":"additional","affiliation":[{"name":"Department of Microbiology, School of Medicine University of Valencia  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3010-4110","authenticated-orcid":false,"given":"David","family":"Navarro","sequence":"additional","affiliation":[{"name":"Microbiology Service Clinic University Hospital, INCLIVA Health Research Institute  Valencia Spain"},{"name":"Department of Microbiology, School of Medicine University of Valencia  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,9]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMcp2009575"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.m1443"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2666-5247(20)30172-5"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra2026131"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-2600(20)30404-5"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.26876"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/hvaa213"},{"issue":"20","key":"e_1_2_9_9_1","first-page":"30721","article-title":"Detection of SARS\u2010CoV\u20102 N\u2010antigen in blood during acute COVID\u201019 provides a sensitive new marker and new testing alternatives","volume":"1198","author":"Hingrat QL","year":"2020","journal-title":"Clin Microbiol Infect"},{"key":"e_1_2_9_10_1","article-title":"Absent or insufficient anti\u2010SARS\u2010CoV\u20102 S antibodies at ICU admission are associated to higher viral loads in plasma, antigenemia and mortality in COVID\u201019 patients","author":"Martin\u2010Vicente M","year":"2021","journal-title":"medRxiv"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2021.05.036"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.12688\/wellcomeopenres.16002.2"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2020.102999"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13613-020-00783-4"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa449"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-020-03398-0"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.27300","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jmv.27300","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.27300","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T17:40:12Z","timestamp":1692985212000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.27300"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9]]},"references-count":15,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022,1]]}},"alternative-id":["10.1002\/jmv.27300"],"URL":"https:\/\/doi.org\/10.1002\/jmv.27300","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,9]]},"assertion":[{"value":"2021-07-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-08-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}