{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T12:27:45Z","timestamp":1763728065683,"version":"3.37.3"},"reference-count":14,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,10,16]],"date-time":"2021-10-16T00:00:00Z","timestamp":1634342400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In Bangladesh, antiretroviral therapy (ART) is provided without screening drug resistance\u2010associated mutations (DRM) among people living with HIV, while DRM might emerge and transmit to the newly infected individual. The present study was aimed to identify DRM among ART\u2010naive clients from an HIV testing and counseling (HTC) center in the initial stages of ART programs. Randomly selected (<jats:italic>n<\/jats:italic>\u2009=\u200964) archived plasma samples were used for the <jats:italic>pol<\/jats:italic> gene amplification and sequencing by sanger technology. Recovered sequences (<jats:italic>n<\/jats:italic>\u2009=\u200910) were genotyped using HIV genotyping tools of NCBI and analyzed using the Stanford University HIV drug resistance database (<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/hivdb.stanford.edu\">hivdb.stanford.edu<\/jats:ext-link>). Various genotypes with a number of DRM were identified in HTC clients, who belonged to different risk groups based on behavioral data. The drug resistance algorithm showed that all samples were fully resistant to tipranavir\/ritonavir drugs except for one intermediate resistance. Despite the small sample size, our understanding from this study warrants an ART policy with a DRM monitoring system for the country.<\/jats:p>","DOI":"10.1002\/jmv.27387","type":"journal-article","created":{"date-parts":[[2021,10,12]],"date-time":"2021-10-12T22:40:54Z","timestamp":1634078454000},"page":"787-790","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Drug resistance pattern among ART\u2010naive clients attending an HIV testing and counseling center in Dhaka, Bangladesh"],"prefix":"10.1002","volume":"94","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0092-9261","authenticated-orcid":false,"given":"Sezanur","family":"Rahman","sequence":"first","affiliation":[{"name":"Virology Laboratory, Infectious Diseases Division International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b) Dhaka Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Md S.","family":"Sarker","sequence":"additional","affiliation":[{"name":"Virology Laboratory, Infectious Diseases Division International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b) Dhaka Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shambhu G.","family":"Aralaguppe","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology, Department of Laboratory Medicine Karolinska Institutet Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Golam","family":"Sarwar","sequence":"additional","affiliation":[{"name":"HIV Programme, Infectious Diseases Division International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b) Dhaka Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sharful I.","family":"Khan","sequence":"additional","affiliation":[{"name":"HIV Programme, Infectious Diseases Division International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b) Dhaka Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6876-0191","authenticated-orcid":false,"given":"Mustafizur","family":"Rahman","sequence":"additional","affiliation":[{"name":"Virology Laboratory, Infectious Diseases Division International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b) Dhaka Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,10,16]]},"reference":[{"unstructured":"National AIDS\/STD Programme2017.Behavioural and Serological Surveillance amongst Key Populations at Risk of HIV in Selected Areas of Bangladesh 2016. Government of the People's Republic of Bangladesh: Ministry of Health and Family Welfare Welfare MoHaF; 2017.","key":"e_1_2_9_2_1"},{"doi-asserted-by":"publisher","key":"e_1_2_9_3_1","DOI":"10.1371\/journal.pone.0079193"},{"doi-asserted-by":"publisher","key":"e_1_2_9_4_1","DOI":"10.1016\/j.ijid.2020.12.037"},{"doi-asserted-by":"publisher","key":"e_1_2_9_5_1","DOI":"10.1179\/2046905513Y.0000000100"},{"doi-asserted-by":"publisher","key":"e_1_2_9_6_1","DOI":"10.5114\/hivar.2020.101794"},{"doi-asserted-by":"publisher","key":"e_1_2_9_7_1","DOI":"10.1001\/jama.296.7.827"},{"doi-asserted-by":"publisher","key":"e_1_2_9_8_1","DOI":"10.1016\/S2352-3018(17)30066-8"},{"unstructured":"National AIDS\/STD Programme.National Anti Retroviral Therapy (NRT) Guidelines Bangladesh. Government of the People's Republic of Bangladesh: Directorate General of Health Services; 2019.","key":"e_1_2_9_9_1"},{"issue":"2","key":"e_1_2_9_10_1","first-page":"77","article-title":"European recommendations for the clinical use of HIV drug resistance testing: 2011 update","volume":"13","author":"Vandamme AM","year":"2011","journal-title":"AIDS Rev"},{"doi-asserted-by":"publisher","key":"e_1_2_9_11_1","DOI":"10.1001\/jama.2012.7961"},{"doi-asserted-by":"publisher","key":"e_1_2_9_12_1","DOI":"10.1371\/journal.pone.0141483"},{"doi-asserted-by":"publisher","key":"e_1_2_9_13_1","DOI":"10.7448\/IAS.18.1.20035"},{"doi-asserted-by":"publisher","key":"e_1_2_9_14_1","DOI":"10.1128\/JVI.02579-07"},{"doi-asserted-by":"publisher","key":"e_1_2_9_15_1","DOI":"10.1089\/aid.2017.0295"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.27387","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jmv.27387","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.27387","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T07:13:56Z","timestamp":1692947636000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.27387"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,16]]},"references-count":14,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2022,2]]}},"alternative-id":["10.1002\/jmv.27387"],"URL":"https:\/\/doi.org\/10.1002\/jmv.27387","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"type":"print","value":"0146-6615"},{"type":"electronic","value":"1096-9071"}],"subject":[],"published":{"date-parts":[[2021,10,16]]},"assertion":[{"value":"2021-05-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}