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We detected the virus in a patient from Araraquara, State of S\u00e3o Paulo, and in patients and in a mosquito pool of <jats:italic>Culex quinquefasciatus<\/jats:italic> from Sinop, State of Mato Grosso. Phylogenetic analysis grouped samples from this study within genotype V, which are closely related to other strains that previously circulated in other parts of the country. 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