{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T15:37:21Z","timestamp":1790869041907,"version":"4.1.0"},"reference-count":12,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,11,2]],"date-time":"2021-11-02T00:00:00Z","timestamp":1635811200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002341","name":"Academy of Finland","doi-asserted-by":"publisher","award":["324236"],"award-info":[{"award-number":["324236"]}],"id":[{"id":"10.13039\/501100002341","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002341","name":"Academy of Finland","doi-asserted-by":"publisher","award":["336472"],"award-info":[{"award-number":["336472"]}],"id":[{"id":"10.13039\/501100002341","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>While most of the spontaneous mutations in the viral genome have no functional, diagnostic, or clinical consequences, some have. In February 2021, we noticed in Southern Finland coronavirus disease 2019 cases where two commercial polymerase chain reaction (PCR) analyses failed to recognize the used N gene target but recognized the other target gene of severe acute respiratory syndrome coronavirus 2. Complete viral genome sequence analysis of the strains revealed several mutations that were not found at that time in public databases. A short 3\u2009bp deletion and three subsequent single nucleotide polymorphisms in the N gene were found exactly at the site where an early published and widely used N gene\u2010based PCR primer is located, explaining the negative results in the N gene PCR. Later the variant strain was identified as a member of the B.1.1.318 Pango lineage that had first been found from Nigerian samples collected in January 2021. This strain shares with the Beta variant the S gene E484K mutation linked to impaired vaccine protection, but differs from this variant in several other ways, for example by deletions in the N gene region. Mutations in the N gene causing diagnostic resistance and on the other hand E484K mutation in the causing altered infectivity warrants careful inspection on virus variants that might get underdiagnosed.<\/jats:p>","DOI":"10.1002\/jmv.27418","type":"journal-article","created":{"date-parts":[[2021,10,26]],"date-time":"2021-10-26T09:25:43Z","timestamp":1635240343000},"page":"1227-1231","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["SARS\u2010CoV\u20102 variant with mutations in N gene affecting detection by widely used PCR primers"],"prefix":"10.1002","volume":"94","author":[{"given":"Pia","family":"Laine","sequence":"first","affiliation":[{"name":"DNA Sequencing and Genomics, Helsinki Institute of Life Science HiLIFE, Institute of Biotechnology University of Helsinki  Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanna","family":"Nihtil\u00e4","sequence":"additional","affiliation":[{"name":"Vita Laboratory  Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ella","family":"Mustanoja","sequence":"additional","affiliation":[{"name":"DNA Sequencing and Genomics, Helsinki Institute of Life Science HiLIFE, Institute of Biotechnology University of Helsinki  Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annina","family":"Lyyski","sequence":"additional","affiliation":[{"name":"DNA Sequencing and Genomics, Helsinki Institute of Life Science HiLIFE, Institute of Biotechnology University of Helsinki  Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Ylinen","sequence":"additional","affiliation":[{"name":"DNA Sequencing and Genomics, Helsinki Institute of Life Science HiLIFE, Institute of Biotechnology University of Helsinki  Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jukka","family":"Hurme","sequence":"additional","affiliation":[{"name":"Vita Laboratory  Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars","family":"Paulin","sequence":"additional","affiliation":[{"name":"DNA Sequencing and Genomics, Helsinki Institute of Life Science HiLIFE, Institute of Biotechnology University of Helsinki  Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sakari","family":"Jokiranta","sequence":"additional","affiliation":[{"name":"Vita Laboratory  Helsinki Finland"},{"name":"Tammer Biolabs  Tampere Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petri","family":"Auvinen","sequence":"additional","affiliation":[{"name":"DNA Sequencing and Genomics, Helsinki Institute of Life Science HiLIFE, Institute of Biotechnology University of Helsinki  Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taru","family":"Meri","sequence":"additional","affiliation":[{"name":"Vita Laboratory  Helsinki Finland"},{"name":"The Molecular and Integrative Biosciences Research Programme (MIBS) University of Helsinki  Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,11,2]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2008-3"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2180-5"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chaos.2020.110018"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.076141.120"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.65962"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2021.4933"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02369-20"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13104-021-05722-5"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2020.25.39.2001650"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01598-20"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsos.200636"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygeno.2020.09.028"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.27418","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jmv.27418","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.27418","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,24]],"date-time":"2023-08-24T12:01:42Z","timestamp":1692878502000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.27418"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,2]]},"references-count":12,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2022,3]]}},"alternative-id":["10.1002\/jmv.27418"],"URL":"https:\/\/doi.org\/10.1002\/jmv.27418","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,2]]},"assertion":[{"value":"2021-09-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}