{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T07:28:40Z","timestamp":1790753320025,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,11,15]],"date-time":"2021-11-15T00:00:00Z","timestamp":1636934400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002941","name":"Departamento de Investigaciones Cient\u00edficas y Tecnol\u00f3gicas, Universidad de Santiago de Chile","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002941","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Due to the COVID\u201019 pandemic, many transport kits have been manufactured to preserve and transport nasopharyngeal swab samples (NPSs) from patients. However, there is no information on the performance of the different virus transport media (VTM) used in COVID\u201019 diagnosis in the population of Santiago de Chile. We compared the RT\u2010qPCR amplification profile of five different viral transport kit mediums, including DNA\/RNA Shield\u2122, NAT, VTM\u2010N, Ezmedlab\u2122, and phosphate\u2010buffered saline (PBS), for NPSs\u00a0from Central Metropolitan Health Service, Santiago, Chile. The DNA\/RNA Shield\u2122 medium showed a better performance in terms of Cq and RFU values for the internal reference RNase P and viral ORF1ab probes. By contrast, the PBS transport medium registered higher Cq values for the viral and reference gene, compared to the other VTM. DNA\/RNA Shield\u2122 shows higher relative fluorescence units (RFUs) and lower Cq values for the reference gene. Collectively, our results suggest that the PBS medium could compromise the sample diagnosis because of its lower RT\u2010qPCR performance. The NAT, Ezmedlab and VTM\u2010N, and DNA\/RNA Shield\u2122 media show acceptable RT\u2010qPCR parameters and, consequently, seem suitable for use in COVID\u201019 diagnosis.<\/jats:p>","DOI":"10.1002\/jmv.27446","type":"journal-article","created":{"date-parts":[[2021,11,10]],"date-time":"2021-11-10T05:49:00Z","timestamp":1636523340000},"page":"1167-1174","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Analysis by real\u2010time PCR of five transport and conservation mediums of nasopharyngeal swab samples to COVID\u201019 diagnosis in Santiago of Chile"],"prefix":"10.1002","volume":"94","author":[{"given":"Carlos","family":"Barrera\u2010Avalos","sequence":"first","affiliation":[{"name":"Centro de Biotecnolog\u00eda Acu\u00edcola, Facultad de Qu\u00edmica y Biolog\u00eda Universidad de Santiago de Chile Santiago 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