{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T17:42:12Z","timestamp":1778002932939,"version":"3.51.4"},"reference-count":27,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,12,14]],"date-time":"2021-12-14T00:00:00Z","timestamp":1639440000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2020M670074ZX"],"award-info":[{"award-number":["2020M670074ZX"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study aimed to explore the application value of two laboratory tests in the diagnosis of Epstein\u2010Barr virus\u2010associated infectious mononucleosis (EBV\u2010IM) in children. From January 2018 to December 2020, 166 patients with EBV\u2010IM were included in this study. Two methods were used in the analysis. The results of both tests were compared and analyzed. The age of onset of EBV\u2010IM is mainly distributed in the range of 0\u20136 years, and no difference by gender is observed. The sensitivity and specificity of EBV testing by PCR were 49.4% and 89.8%, respectively, and the area under the receiver operating characteristic curve (AUC) was 0.714 (0.662\u20130.762). When using the immunofluorescence method to detect EBV antibodies, the two indices with the highest diagnostic efficacy were low\u2010affinity EBV\u2010CA IgG and EBV\u2010CA IgM, and their AUC values were 0.798 (0.751\u20130.840) and 0.663 (0.609\u20130.713), respectively. When combining the two indices for testing, the AUC values of EBV\u2010CA IgM + low\u2010affinity EBV\u2010CA IgG, EBV\u2010DNA+EBV\u2010CA IgM, and EBV\u2010DNA+low\u2010affinity EBV\u2010CA IgG were 0.904 (0.867\u20130.933), 0.768 (0.719\u20130.812), and 0.963 (0.937\u20130.981), respectively. The diagnostic efficacy of the combined EBV\u2010DNA+EBV\u2010CA IgM + low\u2010affinity EBV\u2010CA IgG test was optimal compared with that of a single index or the combination of two indices, with an AUC of 0.999 (0.986\u20131.000;\u00a0<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05), sensitivity of 100%, and specificity of 89.8%. The combined immunofluorescence and real\u2010time PCR methods have high sensitivity and specificity and good application value in the clinical diagnosis of EBV\u2010IM.<\/jats:p>","DOI":"10.1002\/jmv.27490","type":"journal-article","created":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T06:40:25Z","timestamp":1638340825000},"page":"2747-2754","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Analysis of two laboratory tests for determination of EBV\u2010IM in children"],"prefix":"10.1002","volume":"94","author":[{"given":"Zhuo","family":"Li","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine The First Affiliated Hospital of Xi'an Medical University Xi'an Shaanxi China"},{"name":"The National Engineering Research Center for Miniaturized Detection Systems, College of Life Science Northwest University Xi'an Shaanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8152-4719","authenticated-orcid":false,"given":"Xin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine The First Affiliated Hospital of Xi'an Medical University Xi'an Shaanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fahong","family":"Jing","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine The First Affiliated Hospital of Xi'an Medical University Xi'an Shaanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine The First Affiliated Hospital of Xi'an Medical University Xi'an Shaanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine The 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