{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T14:37:58Z","timestamp":1778855878630,"version":"3.51.4"},"reference-count":50,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,12,16]],"date-time":"2021-12-16T00:00:00Z","timestamp":1639612800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014895","name":"Open Philanthropy Project","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100014895","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The coronavirus disease 2019 (COVID\u201019) pandemic has sparked the rapid development of severe acute respiratory syndrome coronavirus 2 (SARS\u2010CoV\u20102) diagnostics. However, emerging variants pose the risk for target dropout and false\u2010negative results secondary to primer\/probe binding site (PBS) mismatches. The Agena MassARRAY\u00ae SARS\u2010CoV\u20102 Panel combines reverse\u2010transcription polymerase chain reaction and matrix\u2010assisted laser desorption\/ionization time\u2010of\u2010flight mass\u2010spectrometry to probe for five targets across\n                    <jats:italic>N<\/jats:italic>\n                    and\n                    <jats:italic>ORF1ab<\/jats:italic>\n                    genes, which provides a robust platform to accommodate PBS mismatches in divergent viruses. Herein, we utilize a deidentified data set of 1262 SARS\u2010CoV\u20102\u2010positive specimens from Mount Sinai Health System (New York City) from December 2020 to April 2021 to evaluate target results and corresponding sequencing data. Overall, the level of PBS mismatches was greater in specimens with target dropout. Of specimens with N3 target dropout, 57% harbored an A28095T substitution that is highly specific for the Alpha (B.1.1.7) variant of concern. These data highlight the benefit of redundancy in target design and the potential for target performance to illuminate the dynamics of circulating SARS\u2010CoV\u20102 variants.\n                  <\/jats:p>","DOI":"10.1002\/jmv.27510","type":"journal-article","created":{"date-parts":[[2021,12,8]],"date-time":"2021-12-08T02:02:52Z","timestamp":1638928972000},"page":"1606-1616","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Robust clinical detection of SARS\u2010CoV\u20102 variants by RT\u2010PCR\/MALDI\u2010TOF multitarget approach"],"prefix":"10.1002","volume":"94","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6217-7264","authenticated-orcid":false,"given":"Matthew M.","family":"Hernandez","sequence":"first","affiliation":[{"name":"Department of Pathology, Molecular, and Cell\u2010Based Medicine Icahn School of Medicine at Mount Sinai New York New York 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