{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T04:33:42Z","timestamp":1793075622685,"version":"4.5.0"},"reference-count":23,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,12,21]],"date-time":"2021-12-21T00:00:00Z","timestamp":1640044800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,12,21]],"date-time":"2021-12-21T00:00:00Z","timestamp":1640044800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The coronavirus disease 2019 (COVID\u201019) is outbreaking all over the world. To help fight this disease, it is necessary to establish an effective and rapid detection method. The nucleocapsid (N) protein of severe acute respiratory syndrome coronavirus 2 (SARS\u2010CoV\u20102) is involved in viral replication, assembly, and immune regulation and plays an important role in the viral life cycle. Moreover, the N protein also could be a diagnostic factor and potential drug target. Therefore, by synthesizing the N gene sequence of SARS\u2010CoV\u20102, constructing the pET\u201028a (+)\u2010N recombinant plasmid, we expressed the N protein in\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    and obtained 15 monoclonal antibody (mAbs) against SARS\u2010CoV\u20102\u2010N protein by the hybridomas and ascites, then an immunochromatographic test strip method detecting N antigen was established. In this study, we obtained 14 high\u2010titer and high\u2010specificity monoclonal antibodies, and the test strips exclusively react with the SARS\u2010CoV\u20102\u2010N protein and no cross\u2010reactivity with other coronavirus and also recognize the recombinant N protein of Delta (B.1.617.2) variant. These mAbs can be used for the early and rapid diagnosis of SARS\u2010CoV\u20102 infection through serological antigen.\n                  <\/jats:p>","DOI":"10.1002\/jmv.27520","type":"journal-article","created":{"date-parts":[[2021,12,13]],"date-time":"2021-12-13T22:08:49Z","timestamp":1639433329000},"page":"1633-1640","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Preparation of highly specific monoclonal antibodies against SARS\u2010CoV\u20102 nucleocapsid protein and the preliminary development of antigen detection test strips"],"prefix":"10.1002","volume":"94","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4596-7026","authenticated-orcid":false,"given":"Chengzuo","family":"Xie","sequence":"first","affiliation":[{"name":"Institute of Parasitic Disease Hangzhou Medical College  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haojie","family":"Ding","sequence":"additional","affiliation":[{"name":"Institute of Parasitic Disease Hangzhou Medical College  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianzu","family":"Ding","sequence":"additional","affiliation":[{"name":"Institute of Parasitic Disease Hangzhou Medical College  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangji","family":"Xue","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Blood Safety Research, Blood Center of Zhejiang Province  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaohong","family":"Lu","sequence":"additional","affiliation":[{"name":"Institute of Parasitic Disease Hangzhou Medical College  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hangjun","family":"Lv","sequence":"additional","affiliation":[{"name":"Institute of Parasitic Disease Hangzhou Medical College  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,12,21]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1213\/ANE.0000000000004845"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/clinpract11040093"},{"issue":"2","key":"e_1_2_9_4_1","first-page":"246","article-title":"[Spike protein in the detection and treatment of novel coronavirus]","volume":"37","author":"Chen Y","year":"2020","journal-title":"Sheng Wu Yi Xue Gong Cheng Xue Za Zhi"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02160-20"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2021.102681"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00461-20"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssensors.0c02561"},{"issue":"3","key":"e_1_2_9_9_1","first-page":"482","article-title":"[Development and application of monoclonal antibodies anti\u2010human lipoprotein\u2010associated phospholipase A2]","volume":"35","author":"Wen X","year":"2019","journal-title":"Sheng Wu Gong Cheng Xue Bao"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-26732-7"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2020.1585"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.25786"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2020.11.025"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2021.01.003"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1086\/422040"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/gzab010"},{"key":"e_1_2_9_17_1","doi-asserted-by":"crossref","first-page":"5","DOI":"10.4103\/ijmr.IJMR_2232_20","article-title":"Development of indigenous IgG ELISA for the detection of anti\u2010SARS\u2010CoV\u20102 IgG","volume":"151","author":"Sapkal G","year":"2020","journal-title":"Indian J Med Res"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2196-x"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.25727"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)13412-5"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2020.00470"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-22072-9"},{"key":"e_1_2_9_23_1","doi-asserted-by":"crossref","unstructured":"Kirola LJNm infections n. 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