{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:11:56Z","timestamp":1772118716511,"version":"3.50.1"},"reference-count":20,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T00:00:00Z","timestamp":1645574400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Saliva is a promising specimen for the detection of viruses that cause upper respiratory infections including severe acute respiratory syndrome coronavirus 2 (SARS\u2010CoV\u20102) due to its cost\u2010effectiveness and noninvasive collection. However, together with intrinsic enzymes and oral microbiota, children's unique dietary habits may introduce substances that interfere with diagnostic testing. To determine whether children's dietary choices impact SARS\u2010CoV\u20102 molecular detection in saliva, we performed a diagnostic study that simulates testing of real\u2010life specimens provided from healthy children (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20095) who self\u2010collected saliva at home before and at 0, 20, and 60\u2009min after eating 20 foods they selected. Each of 72 specimens was split into two volumes and spiked with SARS\u2010CoV\u20102\u2010negative or SARS\u2010CoV\u20102\u2010positive clinical standards before side\u2010by\u2010side testing by reverse\u2010transcription polymerase chain reaction matrix\u2010assisted laser desorption ionization time\u2010of\u2010flight (RT\u2010PCR\/MALDI\u2010TOF) assay. Detection of internal extraction control and SARS\u2010CoV\u20102 nucleic acids was reduced in replicates of saliva collected at 0\u2009min after eating 11 of 20 foods. Interference resolved at 20 and 60\u2009min after eating all foods except hot dogs in one participant. This represented a significant improvement in the detection of nucleic acids compared to saliva collected at 0\u2009min after eating (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.0005). We demonstrate successful detection of viral nucleic acids in saliva self\u2010collected by children before and after eating a variety of foods. Fasting is not required before saliva collection for SARS\u2010CoV\u20102 testing by RT\u2010PCR\/MALDI\u2010TOF, but waiting for 20\u2009min after eating is sufficient for accurate testing. These findings should be considered for SARS\u2010CoV\u20102 testing and broader viral diagnostics in saliva specimens.\n                  <\/jats:p>","DOI":"10.1002\/jmv.27660","type":"journal-article","created":{"date-parts":[[2022,2,16]],"date-time":"2022-02-16T11:01:36Z","timestamp":1645009296000},"page":"2471-2478","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Food for thought: Eating before saliva collection and interference with SARS\u2010CoV\u20102 detection"],"prefix":"10.1002","volume":"94","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6217-7264","authenticated-orcid":false,"given":"Matthew M.","family":"Hernandez","sequence":"first","affiliation":[{"name":"Department of Pathology,\u00a0Molecular, and Cell\u2010Based Medicine Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariawy","family":"Riollano\u2010Cruz","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary C.","family":"Boyle","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Radhika","family":"Banu","sequence":"additional","affiliation":[{"name":"Department of Pathology,\u00a0Molecular, and Cell\u2010Based Medicine Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paras","family":"Shrestha","sequence":"additional","affiliation":[{"name":"Department of Pathology,\u00a0Molecular, and Cell\u2010Based Medicine Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brandon","family":"Gray","sequence":"additional","affiliation":[{"name":"Department of Pathology,\u00a0Molecular, and Cell\u2010Based Medicine Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liyong","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Pathology,\u00a0Molecular, and Cell\u2010Based Medicine Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pathology,\u00a0Molecular, and Cell\u2010Based Medicine Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huanzhi","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Pathology,\u00a0Molecular, and Cell\u2010Based Medicine Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel E.","family":"Paniz\u2010Perez","sequence":"additional","affiliation":[{"name":"Department of Pathology,\u00a0Molecular and Cell\u2010Based Medicine Kids Laboratory and Science Hub, Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul A.","family":"Paniz\u2010Perez","sequence":"additional","affiliation":[{"name":"Department of Pathology,\u00a0Molecular and Cell\u2010Based Medicine Kids Laboratory and Science Hub, Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aryan L.","family":"Rishi","sequence":"additional","affiliation":[{"name":"Department of Pathology,\u00a0Molecular and Cell\u2010Based Medicine Kids Laboratory and Science Hub, Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacob","family":"Dubinsky","sequence":"additional","affiliation":[{"name":"Department of Pathology,\u00a0Molecular and Cell\u2010Based Medicine Kids Laboratory and Science Hub, Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dylan","family":"Dubinsky","sequence":"additional","affiliation":[{"name":"Department of Pathology,\u00a0Molecular and Cell\u2010Based Medicine Kids Laboratory and Science Hub, Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Owen","family":"Dubinsky","sequence":"additional","affiliation":[{"name":"Department of Pathology,\u00a0Molecular and Cell\u2010Based Medicine Kids Laboratory and Science Hub, Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sophie","family":"Baine","sequence":"additional","affiliation":[{"name":"Department of Pathology,\u00a0Molecular and Cell\u2010Based Medicine Kids Laboratory and Science Hub, Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lily","family":"Baine","sequence":"additional","affiliation":[{"name":"Department of Pathology,\u00a0Molecular and Cell\u2010Based Medicine Kids Laboratory and Science Hub, Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suzanne","family":"Arinsburg","sequence":"additional","affiliation":[{"name":"Department of Pathology,\u00a0Molecular, and Cell\u2010Based Medicine Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian","family":"Baine","sequence":"additional","affiliation":[{"name":"Department of Pathology,\u00a0Molecular, and Cell\u2010Based Medicine Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1344-9312","authenticated-orcid":false,"given":"Juan David","family":"Ramirez","sequence":"additional","affiliation":[{"name":"Department of Pathology,\u00a0Molecular, and Cell\u2010Based Medicine Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos","family":"Cordon\u2010Cardo","sequence":"additional","affiliation":[{"name":"Department of Pathology,\u00a0Molecular, and Cell\u2010Based Medicine Icahn School of Medicine at Mount Sinai  New York New York 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